Purpose3rd generation transfer vector
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||40879||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 9462
- Total vector size (bp) 10862
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Growth instructionsGrow bacteria overnight at 30 degrees for good DNA yield.
SpeciesM. musculus (mouse)
Insert Size (bp)1400
Entrez GeneArl13b (a.k.a. A530097K21Rik, A930014M17Rik, Arl2l1, C530009C10Rik, hnn)
- Promoter CMV
/ Fusion Protein
- EGFP (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer WPRE-R (Common Sequencing Primers)
Terms and Licenses
Articles Citing this Plasmid
From Larkins et al (2011), this is the Arl13b-GFP expression virus as described in the Materials and Methods and used in the FRAP experiment shown in Figure 4.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:L13-Arl13bGFP was a gift from Tamara Caspary (Addgene plasmid # 40879 ; http://n2t.net/addgene:40879 ; RRID:Addgene_40879)
For your References section:Arl13b regulates ciliogenesis and the dynamic localization of Shh signaling proteins. Larkins CE, Aviles GD, East MP, Kahn RA, Caspary T. Mol Biol Cell. 2011 Dec;22(23):4694-703. Epub 2011 Oct 5. 10.1091/mbc.E10-12-0994 PubMed 21976698