PurposeExpress FLAG-tagged YAP1 in mammalian cells
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||66853||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepCMV FLAG
Backbone manufacturerKodak (Sigma)
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Alt nameYes-associated protein 1
SpeciesH. sapiens (human)
GenBank ID10413 NM_001130145.2
Entrez GeneYAP1 (a.k.a. COB1, YAP, YAP2, YAP65, YKI)
- Promoter CMV
/ Fusion Protein
- FLAG (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (destroyed during cloning)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer CMV promoter (Common Sequencing Primers)
I subcloned the N-terminal PCR product (BamHI/XhoI) and the XhoI/KpnI fragment from pCMV SPORT YAP1 (Open biosytems) into BglII/KpnI sites of pCMV FLAG.
This is the original pCMV FLAG, with only one FLAG.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pFLAG-YAP1 was a gift from Yutaka Hata (Addgene plasmid # 66853 ; http://n2t.net/addgene:66853 ; RRID:Addgene_66853)
For your References section:A cell-based assay to screen stimulators of the Hippo pathway reveals the inhibitory effect of dobutamine on the YAP-dependent gene transcription. Bao Y, Nakagawa K, Yang Z, Ikeda M, Withanage K, Ishigami-Yuasa M, Okuno Y, Hata S, Nishina H, Hata Y. J Biochem. 2011 Aug;150(2):199-208. doi: 10.1093/jb/mvr063. Epub 2011 May 17. 10.1093/jb/mvr063 PubMed 21586534