pFLAG-YAP1
(Plasmid #66853)

• Purpose: Express FLAG-tagged YAP1 in mammalian cells
• Depositing Lab: Yutaka Hata
• Publication: Bao et al J Biochem. 2011 Aug;150(2):199-208. doi: 10.1093/jb/mvr063. Epub 2011 May 17.

Backbone

• Vector backbone: pCMV FLAG
• Backbone manufacturer: Kodak (Sigma)
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: YAP1
• Alt name: Yes-associated protein 1
• Species: H. sapiens (human)
• GenBank ID: 10413 NM_001130145.2
• Entrez Gene: YAP1 (a.k.a. COB1, YAP, YAP-1, YAP2, YAP65, YKI)
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (destroyed during cloning)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: CMV promoter

Resource Information

• Articles Citing this Plasmid:
  • 11 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

I subcloned the N-terminal PCR product (BamHI/XhoI) and the XhoI/KpnI fragment from pCMV SPORT YAP1 (Open biosytems) into BglII/KpnI sites of pCMV FLAG.
This is the original pCMV FLAG, with only one FLAG.