Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

(Plasmid #34615)


Item Catalog # Description Quantity Price (USD)
Plasmid 34615 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 4800
  • Vector type

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    synthetic TEAD luciferase reporter
  • Alt name
    YAP/TAZ luciferase reporter
  • Alt name
    contains the minimal chicken TnT promoter
  • Insert Size (bp)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site NcoI (not destroyed)
  • 5′ sequencing primer RVprimer3
  • 3′ sequencing primer luciferase-rev
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene sequencing detected a 7 nucleotide insertion at position 107 relative to the author's insert sequence. This results in the loss of a MscI restriction site but does not alter the function of the plasmid.

Please click for general advice and technical tips on how to monitor YAP/TAZ regulation and activity in cell culture by 8xGTIIC lux.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    8xGTIIC-luciferase was a gift from Stefano Piccolo (Addgene plasmid # 34615 ; ; RRID:Addgene_34615)
  • For your References section:

    Role of YAP/TAZ in mechanotransduction. Dupont S, Morsut L, Aragona M, Enzo E, Giulitti S, Cordenonsi M, Zanconato F, Le Digabel J, Forcato M, Bicciato S, Elvassore N, Piccolo S. Nature. 2011 Jun 8;474(7350):179-83. doi: 10.1038/nature10137. 10.1038/nature10137 PubMed 21654799