|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17843||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4700
Vector typeMammalian Expression, Bacterial Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
Alt nameYAP1, YAP65
SpeciesH. sapiens (human)
Insert Size (bp)1500
Entrez GeneYAP1 (a.k.a. COB1, YAP, YAP-1, YAP2, YAP65, YKI)
/ Fusion Protein
- EGFP (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
This clone was generated by my postdoctoral felllow: Xavier Espanel.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEGFP-C3-hYAP1 was a gift from Marius Sudol (Addgene plasmid # 17843 ; http://n2t.net/addgene:17843 ; RRID:Addgene_17843)
For your References section:Akt phosphorylates the Yes-associated protein, YAP, to induce interaction with 14-3-3 and attenuation of p73-mediated apoptosis. Basu S, Totty NF, Irwin MS, Sudol M, Downward J. Mol Cell. 2003 Jan . 11(1):11-23. 10.1016/S1097-2765(02)00776-1 PubMed 12535517