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pCMV-flag S127A YAP
(Plasmid #27370)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 27370 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    YAP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1500
  • Mutation
    S127A ( major phosphorylation site in YAP2)
  • Entrez Gene
    YAP1 (a.k.a. COB1, YAP, YAP2, YAP65, YKI)
  • Tag / Fusion Protein
    • flag (N terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pCMV-Flag-YAP2 was obtained from Marius Sudol.
  • Terms and Licenses
  • Articles Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-flag S127A YAP was a gift from Kunliang Guan (Addgene plasmid # 27370 ; http://n2t.net/addgene:27370 ; RRID:Addgene_27370)
  • For your References section:

    Inactivation of YAP oncoprotein by the Hippo pathway is involved in cell contact inhibition and tissue growth control. Zhao B, Wei X, Li W, Udan RS, Yang Q, Kim J, Xie J, Ikenoue T, Yu J, Li L, Zheng P, Ye K, Chinnaiyan A, Halder G, Lai ZC, Guan KL. Genes Dev. 2007 Nov 1. 21(21):2747-61. 10.1101/gad.1602907 PubMed 17974916