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Rapid blue-light-mediated induction of protein interactions in living cells.
Kennedy MJ, Hughes RM, Peteya LA, Schwartz JW, Ehlers MD, Tucker CL
Nat Methods. 2010 Dec;7(12):973-5. doi: 10.1038/nmeth.1524
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
26866pCRY2PHR-mCherryN1Expresses CRY2(1-498)-mCherry fusion for use in light-inducible protein interaction modules
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26867pCIBN(deltaNLS)-pmGFPExpresses CIBN(1-170, -NLS)-eGFP-CaaX fusion for use in light-inducible protein interaction modules for targeting to the plasma membrane
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26871pCRY2FL(deltaNLS)-mCherryN1Mammalian expression of cytoplasmic CRY2-mCherry
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26888pmCherry-CRY2-CreNExpresses N-terminal fragment of Cre fused to CRY2 for blue light-induced activation of DNA recombination when used with CIBN-CreC. Also expresses mCherry.
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26889pmCherry-CIBN-CreCExpresses C-terminal fragment of Cre fused to CIBN for blue light-induced activation of DNA recombination when used with CRY2-CreN. Also expresses mCherry.
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28240pCIB1(deltaNLS)-pmGFPExpresses CIB1 fused to plasma membrane-localized EGFP
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28243pGal4BD-CRY2Expresses Gal4 DNA Binding Domain fused to CRY2 for blue-light-induced transcription activation
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28244pGal4BD-CRY2PHRExpresses Gal4 DNA Binding Domain fused to CRY2PHR (aa1-498) for blue-light-induced transcription activation
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28245pGal4AD-CIB1Expresses Gal4 Activation Domain fused to CIB1 for blue-light-induced transcription activation
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28246pGal4AD-CIBNExpresses Gal4 Activation Domain fused to CIBN (aa1-170) for blue-light-induced transcription activation
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Antibodies from Article