Rapid blue-light-mediated induction of protein interactions in living cells.
Kennedy MJ, Hughes RM, Peteya LA, Schwartz JW, Ehlers MD, Tucker CL
Nat Methods. 2010 Dec;7(12):973-5. doi: 10.1038/nmeth.1524 PubMed Article
Kennedy MJ, Hughes RM, Peteya LA, Schwartz JW, Ehlers MD, Tucker CL
Nat Methods. 2010 Dec;7(12):973-5. doi: 10.1038/nmeth.1524 PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 26866 | pCRY2PHR-mCherryN1 | Expresses CRY2(1-498)-mCherry fusion for use in light-inducible protein interaction modules |
| 26867 | pCIBN(deltaNLS)-pmGFP | Expresses CIBN(1-170, -NLS)-eGFP-CaaX fusion for use in light-inducible protein interaction modules for targeting to the plasma membrane |
| 26871 | pCRY2FL(deltaNLS)-mCherryN1 | Mammalian expression of cytoplasmic CRY2-mCherry |
| 26888 | pmCherry-CRY2-CreN | Expresses N-terminal fragment of Cre fused to CRY2 for blue light-induced activation of DNA recombination when used with CIBN-CreC. Also expresses mCherry. |
| 26889 | pmCherry-CIBN-CreC | Expresses C-terminal fragment of Cre fused to CIBN for blue light-induced activation of DNA recombination when used with CRY2-CreN. Also expresses mCherry. |
| 28240 | pCIB1(deltaNLS)-pmGFP | Expresses CIB1 fused to plasma membrane-localized EGFP |
| 28243 | pGal4BD-CRY2 | Expresses Gal4 DNA Binding Domain fused to CRY2 for blue-light-induced transcription activation |
| 28244 | pGal4BD-CRY2PHR | Expresses Gal4 DNA Binding Domain fused to CRY2PHR (aa1-498) for blue-light-induced transcription activation |
| 28245 | pGal4AD-CIB1 | Expresses Gal4 Activation Domain fused to CIB1 for blue-light-induced transcription activation |
| 28246 | pGal4AD-CIBN | Expresses Gal4 Activation Domain fused to CIBN (aa1-170) for blue-light-induced transcription activation |