pGal4AD-CIB1
(Plasmid #28245)

• Purpose: Expresses Gal4 Activation Domain fused to CIB1 for blue-light-induced transcription activation
• Depositing Lab: Chandra Tucker
• Publication: Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi: 10.1038/nmeth.1524

Backbone

• Vector backbone: pGADT7rec
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 8000
• Vector type: Yeast Expression
• Selectable markers: LEU2

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Gal4AD
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 1003
• Entrez Gene: CIB1 (a.k.a. AT4G34530, T4L20.110, T4L20_110, cryptochrome-interacting basic-helix-loop-helix 1)
• Entrez Gene: GAL4 (a.k.a. YPL248C, GAL81)
• Promoter: ADH1
• Tag / Fusion Protein:
  • CIB1 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (destroyed during cloning)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry