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PurposeExpresses C-terminal fragment of Cre fused to CIBN for blue light-induced activation of DNA recombination when used with CRY2-CreN. Also expresses mCherry.
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Depositing Lab
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Depositing OrganizationDuke University
Located in United States of America -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 26889 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 26889-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 26889-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepMCherryC1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4722
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCIBN-CreC
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Alt nametruncated CIB1, Cre recombinase C terminal fragment
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SpeciesA. thaliana (mustard weed)
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Insert Size (bp)1274
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Mutationaa 1-170 of CIB1, aa 106-343 of Cre
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Sac I (not destroyed)
- 3′ cloning site Xma I (not destroyed)
- 5′ sequencing primer n/a
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 26889-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 26889-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pmCherry-CIBN-CreC was a gift from Chandra Tucker (Addgene plasmid # 26889 ; http://n2t.net/addgene:26889 ; RRID:Addgene_26889) -
For your References section:
Rapid blue-light-mediated induction of protein interactions in living cells. Kennedy MJ, Hughes RM, Peteya LA, Schwartz JW, Ehlers MD, Tucker CL. Nat Methods. 2010 Dec;7(12):973-5. doi: 10.1038/nmeth.1524 10.1038/nmeth.1524 PubMed 21037589
Map uploaded by the depositor.
