|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||31784||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 7000
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth instructionsSlow growing plasmids, grow at least 18 hours.
Copy numberLow Copy
SpeciesH. sapiens (human)
Insert Size (bp)4173
Entrez GeneMET (a.k.a. AUTS9, DA11, DFNB97, HGFR, RCCP2, c-Met)
- Promoter EF1alpha
- Cloning method Gateway Cloning
- 5′ sequencing primer EF-1a-Fwd (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
This plasmid is in the pT3-EF1a vector which contains loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid cannot not used with Cre for in vivo studies.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pT3-EF1a-c-Met was a gift from Xin Chen (Addgene plasmid # 31784 ; http://n2t.net/addgene:31784 ; RRID:Addgene_31784)
For your References section:Distinct pathways of genomic progression to benign and malignant tumors of the liver. Tward AD, Jones KD, Yant S, Cheung ST, Fan ST, Chen X, Kay MA, Wang R, Bishop JM. Proc Natl Acad Sci U S A. 2007 Sep 11;104(37):14771-6. Epub 2007 Sep 4. 10.1073/pnas.0706578104 PubMed 17785413