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Bruce Conklin Lab Plasmids


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Title Authors Publication
Isolation of single-base genome-edited human iPS cells without antibiotic selection. Miyaoka Y, Chan AH, Judge LM, Yoo J, Huang M, Nguyen TD, Lizarraga PP, So PL, Conklin BR Nat Methods. 2014 Mar;11(3):291-3. doi: 10.1038/nmeth.2840. Epub 2014 Feb 9.
Alternative splicing regulates mouse embryonic stem cell pluripotency and differentiation. Salomonis N, Schlieve CR, Pereira L, Wahlquist C, Colas A, Zambon AC, Vranizan K, Spindler MJ, Pico AR, Cline MS, Clark TA, Williams A, Blume JE, Samal E, Mercola M, Merrill BJ, Conklin BR Proc Natl Acad Sci U S A. 2010 Jun 8;107(23):10514-9. Epub 2010 May 24.
Osteoblast expression of an engineered Gs-coupled receptor dramatically increases bone mass. Hsiao EC, Boudignon BM, Chang WC, Bencsik M, Peng J, Nguyen TD, Manalac C, Halloran BP, Conklin BR, Nissenson RA Proc Natl Acad Sci U S A. 2008 Jan 29. 105(4):1209-14.
Engineered G protein coupled receptors reveal independent regulation of internalization, desensitization and acute signaling. Scearce-Levie K, Lieberman MD, Elliott HH, Conklin BR BMC Biol. 2005 . 3():3.
Controlling signaling with a specifically designed Gi-coupled receptor. Coward P, Wada HG, Falk MS, Chan SD, Meng F, Akil H, Conklin BR. Proc Natl Acad Sci U S A. 1998 Jan 6;95(1):352-7.
Carboxyl-terminal mutations of Gq alpha and Gs alpha that alter the fidelity of receptor activation. Conklin BR, Herzmark P, Ishida S, Voyno-Yasenetskaya TA, Sun Y, Farfel Z, Bourne HR Mol Pharmacol. 1996 Oct . 50(4):885-90.
Substitution of three amino acids switches receptor specificity of Gq alpha to that of Gi alpha. Conklin BR, Farfel Z, Lustig KD, Julius D, Bourne HR Nature. 1993 May 20. 363(6426):274-6.
Systematic quantification of HDR and NHEJ reveals effects of locus, nuclease, and cell type on genome-editing Miyaoka et al Scientific Reports 6, Article number: 23549 (2016)
RO1 (unpublished)
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
16312pUNIV-SIG-5HTR4D100Ah5HT4b Serotonin Receptor (Rs1 D100A RASSL) (Homo sapiens)Mammalian ExpressionOsteoblast expression of an engineered Gs-coupled receptor dramatically increases bone mass. Proc Natl Acad Sci U S A. 2008 Jan 29. 105(4):1209-14. pUNIV HTR4 5-HT4, 5-HT4R Add to Cart
16313pUHG10.3-TetO-Rs1h5HT4b Serotonin Receptor (Rs1 D100A RASSL) (Homo sapiens)Mammalian ExpressionOsteoblast expression of an engineered Gs-coupled receptor dramatically increases bone mass. Proc Natl Acad Sci U S A. 2008 Jan 29. 105(4):1209-14. pUHG10-3 RS1 RP1-245G19.1, RS, XLRS1 Add to Cart
24429pcDNA3-RogRASSL (Homo sapiens)Mammalian ExpressionEngineered G protein coupled receptors reveal independent regulation of internalization, desensitization and acute signaling. BMC Biol. 2005 . 3():3. pcDNA3 OPRK1 K-OR-1, KOR, KOR-1, OPRK Add to Cart
24498qs5Gq alpha (Mus musculus)Mammalian ExpressionCarboxyl-terminal mutations of Gq alpha and Gs alpha that alter the fidelity of receptor activation. Mol Pharmacol. 1996 Oct . 50(4):885-90. pcDNA1 Gnaq 1110005L02Rik, 6230401I02Rik, AA408290, AW060788, Dsk1, Dsk10, Galphaq, Gq, GqI Add to Cart
24499sq5Gs alpha (Rattus norvegicus)Mammalian ExpressionCarboxyl-terminal mutations of Gq alpha and Gs alpha that alter the fidelity of receptor activation. Mol Pharmacol. 1996 Oct . 50(4):885-90. pcDNA1 Gnas Gnas1, Gnpas, Nesp55 Add to Cart
24500qo5Gq alpha (Mus musculus)Mammalian ExpressionSubstitution of three amino acids switches receptor specificity of Gq alpha to that of Gi alpha. Nature. 1993 May 20. 363(6426):274-6. pcDNA1 Gnaq 1110005L02Rik, 6230401I02Rik, AA408290, AW060788, Dsk1, Dsk10, Galphaq, Gq, GqI Add to Cart
24501qi5Gq alpha (Mus musculus)Mammalian ExpressionSubstitution of three amino acids switches receptor specificity of Gq alpha to that of Gi alpha. Nature. 1993 May 20. 363(6426):274-6. pcDNA1 Gnaq 1110005L02Rik, 6230401I02Rik, AA408290, AW060788, Dsk1, Dsk10, Galphaq, Gq, GqI Add to Cart
24502q4wtGq alpha (Mus musculus)Mammalian ExpressionSubstitution of three amino acids switches receptor specificity of Gq alpha to that of Gi alpha. Nature. 1993 May 20. 363(6426):274-6. pcDNA1 Add to Cart
24503pcDNA3-Ro2RASSL Ro2 (Homo sapiens)Mammalian ExpressionControlling signaling with a specifically designed Gi-coupled receptor. Proc Natl Acad Sci U S A. 1998 Jan 6;95(1):352-7. pcDNA3 OPRK1 K-OR-1, KOR, KOR-1, OPRK Add to Cart
24504pcDNA3-Ro1RASSL Ro1 (Homo sapiens)Mammalian Expression RO1 (unpublished) pcDNA3 OPRK1 K-OR-1, KOR, KOR-1, OPRK Add to Cart
25867qz5Gq alpha (Mus musculus)Mammalian ExpressionSubstitution of three amino acids switches receptor specificity of Gq alpha to that of Gi alpha. Nature. 1993 May 20. 363(6426):274-6. pcDNA1 Gnaq 1110005L02Rik, 6230401I02Rik, AA408290, AW060788, Dsk1, Dsk10, Galphaq, Gq, GqI Add to Cart
31845pSicoR-Ef1a-mCh-PuroMammalian Expression, Lentiviral, RNAi, Cre/LoxAlternative splicing regulates mouse embryonic stem cell pluripotency and differentiation. Proc Natl Acad Sci U S A. 2010 Jun 8;107(23):10514-9. Epub 2010 May 24. pSicoR-mCH Add to Cart
31847pSicoR-Ef1a-mChMammalian Expression, Lentiviral, RNAi, Cre/LoxAlternative splicing regulates mouse embryonic stem cell pluripotency and differentiation. Proc Natl Acad Sci U S A. 2010 Jun 8;107(23):10514-9. Epub 2010 May 24. pSicoR-mCH Add to Cart
31848pSicoR-Ef1a-mCh-Puro-GFPiGFP RNAiMammalian Expression, Lentiviral, RNAi, Cre/LoxAlternative splicing regulates mouse embryonic stem cell pluripotency and differentiation. Proc Natl Acad Sci U S A. 2010 Jun 8;107(23):10514-9. Epub 2010 May 24. pSicoR-Ef1a-mCH-Puro Add to Cart
31849pSicoR-Ef1a-mCh-GFPiGFP RNAiMammalian Expression, Lentiviral, RNAi, Cre/LoxAlternative splicing regulates mouse embryonic stem cell pluripotency and differentiation. Proc Natl Acad Sci U S A. 2010 Jun 8;107(23):10514-9. Epub 2010 May 24. pSicoR-Ef1a-mCh Add to Cart
31850pSicoR-Ef1a-mCh-Puro-LuciLuciferase RNAiMammalian Expression, Lentiviral, RNAi, Cre/LoxAlternative splicing regulates mouse embryonic stem cell pluripotency and differentiation. Proc Natl Acad Sci U S A. 2010 Jun 8;107(23):10514-9. Epub 2010 May 24. pSicoR-Ef1a-mCH-Puro Add to Cart
49449MR015-PHOX2BRightTALEN_NNTALEN (Other)Mammalian Expression, TALENIsolation of single-base genome-edited human iPS cells without antibiotic selection. Nat Methods. 2014 Mar;11(3):291-3. doi: 10.1038/nmeth.2840. Epub 2014 Feb 9. Right TALEN for PHOX2B with NN MR015 Add to Cart
49450MR015-PHOX2BLeftTALEN_NNTALEN (Other)Mammalian Expression, TALENIsolation of single-base genome-edited human iPS cells without antibiotic selection. Nat Methods. 2014 Mar;11(3):291-3. doi: 10.1038/nmeth.2840. Epub 2014 Feb 9. Left TALEN for PHOX2B with NN MR015 Add to Cart
49451MR015-PKP2 Left TALEN_NNTALEN (Other)Mammalian Expression, TALENIsolation of single-base genome-edited human iPS cells without antibiotic selection. Nat Methods. 2014 Mar;11(3):291-3. doi: 10.1038/nmeth.2840. Epub 2014 Feb 9. Left TALEN for PKP2 with NN MR015 Add to Cart
49452MR015-PKP2 Right TALEN_NNTALEN (Other)Mammalian Expression, TALENIsolation of single-base genome-edited human iPS cells without antibiotic selection. Nat Methods. 2014 Mar;11(3):291-3. doi: 10.1038/nmeth.2840. Epub 2014 Feb 9. Right TALEN for PKP2 with NN MR015 Add to Cart
49453MR015-PRKAG2 Left TALENTALEN (Other)Mammalian Expression, TALENIsolation of single-base genome-edited human iPS cells without antibiotic selection. Nat Methods. 2014 Mar;11(3):291-3. doi: 10.1038/nmeth.2840. Epub 2014 Feb 9. Left TALEN for PRKAG2 MR015 Add to Cart
49454MR015-PRKAG2 Right TALENTALEN (Other)Mammalian Expression, TALENIsolation of single-base genome-edited human iPS cells without antibiotic selection. Nat Methods. 2014 Mar;11(3):291-3. doi: 10.1038/nmeth.2840. Epub 2014 Feb 9. Right TALEN for PRKAG2 MR015 Add to Cart
49465MR015-BAG3 Left TALENTALEN (Other)Mammalian Expression, TALENIsolation of single-base genome-edited human iPS cells without antibiotic selection. Nat Methods. 2014 Mar;11(3):291-3. doi: 10.1038/nmeth.2840. Epub 2014 Feb 9. Left TALEN for BAG3 MR015 Add to Cart
49466MR015-BAG3 Right TALENTALEN (Other)Mammalian Expression, TALENIsolation of single-base genome-edited human iPS cells without antibiotic selection. Nat Methods. 2014 Mar;11(3):291-3. doi: 10.1038/nmeth.2840. Epub 2014 Feb 9. Right TALEN for BAG3 MR015 Add to Cart
49477MR015-PHOX2BLeftTALEN_NKTALEN (Other)Mammalian Expression, TALENIsolation of single-base genome-edited human iPS cells without antibiotic selection. Nat Methods. 2014 Mar;11(3):291-3. doi: 10.1038/nmeth.2840. Epub 2014 Feb 9. Left TALEN for PHOX2B with NK MR015 Add to Cart
49478MR015-PHOX2BRightTALEN_NKTALEN (Other)Mammalian Expression, TALENIsolation of single-base genome-edited human iPS cells without antibiotic selection. Nat Methods. 2014 Mar;11(3):291-3. doi: 10.1038/nmeth.2840. Epub 2014 Feb 9. Right TALEN for PHOX2B with NK MR015 Add to Cart
49479MR015-PKP2 Left TALEN_NKTALEN (Other)Mammalian Expression, TALENIsolation of single-base genome-edited human iPS cells without antibiotic selection. Nat Methods. 2014 Mar;11(3):291-3. doi: 10.1038/nmeth.2840. Epub 2014 Feb 9. Left TALEN for PKP2 with NK MR015 Add to Cart
49480MR015-PKP2 Right TALEN_NKTALEN (Other)Mammalian Expression, TALENIsolation of single-base genome-edited human iPS cells without antibiotic selection. Nat Methods. 2014 Mar;11(3):291-3. doi: 10.1038/nmeth.2840. Epub 2014 Feb 9. Right TALEN for PKP2 with NK MR015 Add to Cart
60900pXCas9H840AMammalian Expression, CRISPRSystematic quantification of HDR and NHEJ reveals effects of locus, nuclease, and cell type on genome-editing Scientific Reports 6, Article number: 23549 (2016) Dual Expression Vector for Cas9 H840A nickase and gRNA pUC ori vector
  • Tag / Fusion Protein
    • 3XFLAG (N terminal on backbone)
  • Add to Cart
    60901pXFokI-dCas9Mammalian Expression, CRISPRSystematic quantification of HDR and NHEJ reveals effects of locus, nuclease, and cell type on genome-editing Scientific Reports 6, Article number: 23549 (2016) Dual Expression Vector for FokI-dCas9 and gRNA pUC ori vector
  • Tag / Fusion Protein
    • 3XFLAG (C terminal on backbone)
  • Add to Cart