A CRISPR-Cpf1 system for efficient genome editing and transcriptional repression in plants.
Tang X, Lowder LG, Zhang T, Malzahn AA, Zheng X, Voytas DF, Zhong Z, Chen Y, Ren Q, Li Q, Kirkland ER, Zhang Y, Qi Y
Nat Plants. 2017 Feb 17;3:17018. doi: 10.1038/nplants.2017.18.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 86196 | pYPQ141-ZmUbi-RZ-As | AsCpf1 gRNA entry plasmid using Zea mays Ubi promoter and ribozyme processing |
| 86197 | pYPQ141-ZmUbi-RZ-Lb | LbCpf1 Gateway gRNA entry plasmid using Zea mays Ubi promoter and ribozyme processing |
| 86198 | pYPQ202 | Multisite Gateway T-DNA entry plasmid (attR1 & attR2); AtUBQ10 promoter and Hygromiycin resistance marker |
| 86207 | pYPQ203 (pMDC32-Ubi1) | Multisite Gateway T-DNA entry plasmid (attR1 & attR2); Zea mays Ubi1 promoter and Hygromiycin resistance marker |
| 86208 | pYPQ220 (AsCpf1) | AsCpf1 Gateway entry plasmid |
| 86209 | pYPQ223 (dAsCpf1-SRDX) | Deactivated AsCpf1-SRDX repressor fusion; transcriptional repressor dCpf1 Gateway entry plasmid |
| 86210 | pYPQ230 (LbCpf1) | LbCpf1 Gateway entry plasmid |
| 86211 | pYPQ233 (dLbCpf1-SRDX) | Deactivated LbCpf1-SRDX repressor fusion; transcriptional repressor dCpf1 Gateway entry plasmid |