Purpose(Empty Backbone) Multisite Gateway T-DNA entry plasmid (attR1 & attR2); Zea mays Ubi1 promoter and Hygromiycin resistance marker
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||86207||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Modifications to backboneArabidopsis ubiquitin 10 (AtUbi10) promoter added to pYPQ202 backbone
Vector typePlant Expression ; Gateway compatible attR1-attR2 destination vector
- Promoter Maize ubiquitin 1 (ZmUbi1)
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Kanamycin, 25 & 50 μg/mL
Growth Strain(s)ccdB Survival
Copy numberHigh Copy
- Cloning method Restriction Enzyme
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Note: Addgene's quality control sequencing finds a 1.2kb transposable element upstream of the Kanamycin resistance cassette. This inserted sequence does not affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pYPQ203 (pMDC32-Ubi1) was a gift from Yiping Qi (Addgene plasmid # 86207 ; http://n2t.net/addgene:86207 ; RRID:Addgene_86207)
For your References section:A CRISPR-Cpf1 system for efficient genome editing and transcriptional repression in plants. Tang X, Lowder LG, Zhang T, Malzahn AA, Zheng X, Voytas DF, Zhong Z, Chen Y, Ren Q, Li Q, Kirkland ER, Zhang Y, Qi Y. Nat Plants. 2017 Feb 17;3:17018. doi: 10.1038/nplants.2017.18. 10.1038/nplants.2017.18 PubMed 28211909