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CRISPR Interference Efficiently Induces Specific and Reversible Gene Silencing in Human iPSCs.
Mandegar MA, Huebsch N, Frolov EB, Shin E, Truong A, Olvera MP, Chan AH, Miyaoka Y, Holmes K, Spencer CI, Judge LM, Gordon DE, Eskildsen TV, Villalta JE, Horlbeck MA, Gilbert LA, Krogan NJ, Sheikh SP, Weissman JS, Qi LS, So PL, Conklin BR
Cell Stem Cell. 2016 Apr 7;18(4):541-53. doi: 10.1016/j.stem.2016.01.022. Epub 2016 Mar 10.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
73497pAAVS1-NDi-CRISPRi (Gen1)Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus with mCherry marker
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73498pAAVS1-NDi-CRISPRi (Gen2)Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus
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73499pAAVS1-NC-CRISPRi (Gen3)Constitutive CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus
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73500pAAVS1-PDi-CRISPRnDox-inducible CRISPR nuclease (CRISPRn) knock in construct into the AAVS1 locus
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73501pgRNA-CKBgRNA expression vector (with mKate2)
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73503pAAVS1-PC-GCaMP6fGCaMP6f knock in construct into the AAVS1 locus
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