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Repurposing the CRISPR-Cas9 system for targeted DNA methylation.
Vojta A, Dobrinic P, Tadic V, Bockor L, Korac P, Julg B, Klasic M, Zoldos V
Nucleic Acids Res. 2016 Mar 11. pii: gkw159.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
71666pdCas9-DNMT3A-EGFPExpression of human codon-optimized dCas9-DNMT3A fusion with T2A-EGFP for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA
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71667pdCas9-DNMT3A-PuroRExpression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA
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71684pdCas9-DNMT3A-PuroR (ANV)Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR; cloning backbone for sgRNA
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71685pdCas9-DNMT3A-EGFP (ANV)Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-EGFP; cloning backbone for sgRNA
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71828pdCas9-DNMT3A-PuroR_BACH2-sgRNA8Expression of dCas9-DNMT3A fusion with T2A-PuroR and specific sgRNA for targeted DNA methylation of BACH2 promoter in human cells; for use as a control
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71829pdCas9-DNMT3A-PuroR_BACH2-sgRNA8 (ANV)Expression of mutant dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR and specific sgRNA for the human BACH2 promoter; for use as a control
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71830pdCas9-DNMT3A-PuroR_hNTExpression of dCas9-DNMT3A fusion with T2A-PuroR and non-targeting control sgRNA for use in human cells
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74407pdCas9-DNMT3A-PuroR_v2Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR (v2) for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA
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74408pdCas9-DNMT3A-PuroR (ANV)_v2Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR (v2); cloning backbone for sgRNA
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Antibodies from Article