Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Repurposing the CRISPR-Cas9 system for targeted DNA methylation.
Vojta A, Dobrinic P, Tadic V, Bockor L, Korac P, Julg B, Klasic M, Zoldos V
Nucleic Acids Res. 2016 Mar 11. pii: gkw159.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
71666pdCas9-DNMT3A-EGFPExpression of human codon-optimized dCas9-DNMT3A fusion with T2A-EGFP for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA
Loading...
71667pdCas9-DNMT3A-PuroRExpression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA
Loading...
71684pdCas9-DNMT3A-PuroR (ANV)Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR; cloning backbone for sgRNA
Loading...
71685pdCas9-DNMT3A-EGFP (ANV)Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-EGFP; cloning backbone for sgRNA
Loading...
71828pdCas9-DNMT3A-PuroR_BACH2-sgRNA8Expression of dCas9-DNMT3A fusion with T2A-PuroR and specific sgRNA for targeted DNA methylation of BACH2 promoter in human cells; for use as a control
Loading...
71829pdCas9-DNMT3A-PuroR_BACH2-sgRNA8 (ANV)Expression of mutant dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR and specific sgRNA for the human BACH2 promoter; for use as a control
Loading...
71830pdCas9-DNMT3A-PuroR_hNTExpression of dCas9-DNMT3A fusion with T2A-PuroR and non-targeting control sgRNA for use in human cells
Loading...
74407pdCas9-DNMT3A-PuroR_v2Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR (v2) for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA
Loading...
74408pdCas9-DNMT3A-PuroR (ANV)_v2Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR (v2); cloning backbone for sgRNA
Loading...