Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Addgene is open for ordering and depositing; find up-to-date details here. To learn more about how we are supporting COVID-19 research and to find related plasmids, check out our COVID-19 and Coronavirus Plasmids & Resources page.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

A versatile viral system for expression and depletion of proteins in mammalian cells.
Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, Campisi J, Yaswen P, Cooper PK, Kaufman PD.
PLoS One. 2009 Aug 6;4(8):e6529.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
17291pLenti CMV/TO Hygro DEST (693-2)3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, Hygro
Loading...
17292pLenti CMV/TO Neo DEST (685-3)3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, Neo
Loading...
17293pLenti CMV/TO Puro DEST (670-1)3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, Puro
Loading...
17294pLenti CMV/TO Zeo DEST (644-1)3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, Zeo
Loading...
17295pLenti X2 Hygro DEST (w17-1)3rd gen lentiviral Gateway destination vector, RNAi, 3’LTR insertion, Hygro
Loading...
17296pLenti X2 Puro DEST (w16-1)3rd gen lentiviral Gateway destination vector, RNAi, 3’LTR insertion, Puro
Loading...
17297pLenti X1 Puro DEST (694-6)3rd gen lentiviral promoter-less Gateway destination vector, Puro
Loading...
17338pENTR/pSUPER+ (575-1)Gateway entry vector for shRNA expression under H1 promoter
Loading...
17386pQCXP CMV/TO DEST (w360-1)Retroviral Gateway destination vector, Expression, CMV/TO promoter, Puro
Loading...
17387pENTR/pSM2(U6) (w51-1)Gateway entry vector for miRNA expression under U6 promoter
Loading...
17388pENTR/pSM2(CMV/TO) (w55-1)Gateway entry vector for miRNA expression under CMV/TO promoter
Loading...
17389pENTR/pSM2(CMV) (w57-1)Gateway entry vector for miRNA expression under CMV promoter
Loading...
17390pLenti X2 Neo DEST (w18-1)3rd gen lentiviral Gateway destination vector, RNAi, 3’LTR insertion, Neo
Loading...
17391pLenti X2 Blast DEST (w46-2)3rd gen lentiviral Gateway destination vector, RNAi, 3’LTR insertion, Blast
Loading...
17392pLenti CMV Neo DEST (705-1)3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Neo
Loading...
17393pQCXP CMV/TO empty (w368-1)Retroviral Empty control vector, CMV/TO promoter, Puro
Loading...
17394pQCXIH CMV/TO DEST (w382-1)Retroviral Gateway destination vector, Expression, CMV/TO promoter, Hygro
Loading...
17396pENTR4-GFP-C1 (w392-1)Gateway entry vector for N-term eGFP fusion, frame C1
Loading...
17397pENTR4-GFP-C3 (w393-1)Gateway entry vector for N-term eGFP fusion, frame C3
Loading...
17398pENTR1A no ccDB (w48-1)Gateway entry 1A vector, no ccDB gene
Loading...
17399pQCXIN X2 DEST (w310-1)Retroviral Gateway destination vector, RNAi, 3’LTR insertion, Neo
Loading...
17400pQCXIB CMV/TO DEST (w320-1)Retroviral Gateway destination vector, Expression, CMV/TO promoter, Blast 
Loading...
17401pQCXIZ CMV/TO DEST (w321-2)Retroviral Gateway destination vector, Expression, CMV/TO promoter, Zeo
Loading...
17402pENTR1A-NTAP-A (w322-1)Gateway entry vector for NTAP (N-terminal affinity purification), frame A
Loading...
17403pENTR1A-NTAP-B (w323-1)Gateway entry vector for NTAP (N-terminal affinity purification), frame B
Loading...
17404pENTR1A-NTAP-C (w324-1)Gateway entry vector for NTAP (N-terminal affinity purification), frame C
Loading...
17407pENTR4-V5-2-Ubc9 (w345-2)Gateway entry vector for N-term V5 tag, frame B and Ubc9 C-term
Loading...
17413pENTR1A-FKBP12DD C-term (w350-2)Gateway entry vector for Degron, C-term
Loading...
17414pENTR4-FKBP12DD N-term A (B3-1)Gateway entry vector for Degron, N-term, frame A
Loading...
17415pENTR4-FKBP12DD N-term B (w385-4)Gateway entry vector for Degron, N-term, frame B
Loading...
17416pENTR4-FKBP12DD N-term C (w385-10)Gateway entry vector for Degron, N-term, frame C
Loading...
17423pENTR4-FLAG (w210-2)Gateway entry vector for N-term FLAG tag
Loading...
17424pENTR4 no ccDB (686-1)Gateway entry vector, no ccDB gene
Loading...
17425pENTR4-V5 (w71-3)Gateway entry vector for N-term V5 tag, frame A
Loading...
17426pENTR4-V5-2 (w234-1)Gateway entry vector for N-term V5 tag, frame B
Loading...
17427pEF-ENTR A (696-6)Gateway entry vector with EF1-a promoter, V5-His C-term, frame A
Loading...
17428pEF-ENTR B (708-1)Gateway entry vector with EF1-a promoter, V5-His C-term, frame B
Loading...
17429pEF-ENTR C (709-1)Gateway entry vector with EF1-a promoter, V5-His C-term, frame C 
Loading...
17430pEF-ENTR-LacZ (710-1)Gateway entry vector with EF1-a promoter, V5-His C-term, Lac Z
Loading...
17431pLenti CMV/TO GFP-Zeo DEST (719-1)3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, GFP-Zeo
Loading...
17432pLenti CMV/TO DEST (775-1)3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter
Loading...
17445pLenti CMV GFP Blast (659-1)3rd gen lentiviral eGFP expression vector, CMV promoter, Blast
Loading...
17446pLenti CMV GFP Hygro (656-4)3rd gen lentiviral eGFP expression vector, CMV promoter, Hygro
Loading...
17447pLenti CMV GFP Neo (657-2)3rd gen lentiviral eGFP expression vector, CMV promoter, Neo
Loading...
17448pLenti CMV GFP Puro (658-5)3rd gen lentiviral eGFP expression vector, CMV promoter, Puro
Loading...
17449pLenti CMV GFP Zeo (637-7)3rd gen lentiviral eGFP expression vector, CMV promoter, Zeo
Loading...
17451pLenti CMV Blast DEST (706-1)3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Blast 
Loading...
17452pLenti CMV Puro DEST (w118-1)3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Puro
Loading...
17453pENTR/pTER+ (430-1)Gateway entry vector for shRNA expression under H1/TO promoter
Loading...
17454pLenti CMV Hygro DEST (w117-1)3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Hygro
Loading...
17455pL106P-LUC-ENTR1A(w451-1)Gateway entry vector, Degron N-term Luciferase
Loading...
17470pENTR/pTER shEGFP #1 (628-2)Gateway entry vector for EGFPshRNA#1 under H1/TO promoter
Loading...
17471pENTR/pTER shEGFP #2 (728-2)Gateway entry vector for EGFPshRNA#2 under H1/TO promoter
Loading...
17472pENTR/pTER shLUC (w177-1)Gateway entry vector for Firefly Luciferase shRNA under H1/TO promoter
Loading...
17473pENTR-LUC (w158-1)Gateway entry vector, Firefly Luciferase
Loading...
17474pQCXIP X2 DEST (w270-1)Retroviral Gateway destination vector, RNAi, 3’LTR insertion, Puro
Loading...
17475pQCXIB CMV/TO LUC (w431-1)Retroviral Luciferase Expression vector, CMV/TO promoter, Blast 
Loading...
17476pQCXP CMV/TO LUC (w432-1)Retroviral Luciferase expression vector, CMV/TO promoter, Puro 
Loading...
17477pLenti CMV Puro LUC (w168-1)3rd gen lentiviral Luciferase expression vector, CMV promoter, Puro
Loading...
17478pLenti X2 Neo empty (w36-1)3rd gen lentiviral RNAi empty control vector, 3’LTR insertion, Neo 
Loading...
17479pLenti X2 Neo/pTER shEGFP#1 (w21-2)3rd gen lentiviral eGFP shRNA #1 (H1/TO promoter), 3’LTR insertion, Neo 
Loading...
17480pLenti X2 Neo/pTER shEGFP#2 (w22-2)3rd gen lentiviral eGFP shRNA #2 (H1/TO promoter), 3’LTR insertion, Neo 
Loading...
17481pLenti CMV/TO eGFP Puro (w159-1)3rd gen lentiviral eGFP expression vector, CMV/TO promoter, Puro 
Loading...
17482pLenti CMV/TO Puro empty (w175-1)3rd gen lentiviral empty control vector, CMV/TO promoter, Puro
Loading...
17483pLenti X2 Neo/pTER shLUC (w181-1)3rd gen lentiviral Luciferase shRNA (H1/TO promoter), 3’LTR insertion, Neo 
Loading...
17484pLenti CMV/TO Hygro empty (w214-1)3rd gen lentiviral empty control vector, CMV/TO promoter, Hygro 
Loading...
17485pLenti CMV/TO Neo empty (w215-1)3rd gen lentiviral empty control vector, CMV/TO promoter, Neo
Loading...
17486pLenti CMV Blast empty (w263-1)3rd gen lentiviral empty control vector, CMV promoter, Blast 
Loading...
17487pQCXIB empty (w335-1)Retroviral Empty control vector, CMV/TO promoter, Blast 
Loading...
17488pQCXIN X2/pSM2(CMV/TO) empty (w334-1)Retroviral Empty control vector, miRNA (CMV/TO), 3’LTR insertion, Neo 
Loading...
17489pQCXIN X2/pTER shLUC (w347-1)Retroviral Luciferase shRNA (H1/TO promoter), 3’LTR insertion, Neo
Loading...
17491pQCXIP X2/pTER shLUC (w454-1)Retroviral Luciferase shRNA (H1/TO promoter), 3’LTR insertion, Puro
Loading...
17492pLenti CMV TetR Blast (716-1)3rd gen lentiviral Tetracycline repressor expression vector, CMV promoter, Blast 
Loading...
17740pQCXP CMV/TO V5-LUC (w476-1)Retroviral V5-Luciferase expression vector, CMV/TO promoter, Puro 
Loading...
17741pENTR4-GST 6P-1 (w487-1)Gateway entry vector for N-term GST fusion, frame 6P-1
Loading...
19066pLenti PGK Hygro DEST (w530-1)3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Hygro
Loading...
19067pLenti PGK Neo DEST (w531-1)3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Neo
Loading...
19068pLenti PGK Puro DEST (w529-2)3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Puro
Loading...
19069pLenti PGK GFP Blast (w510-5)3rd gen lentiviral eGFP expression vector, PGK promoter, Blast
Loading...
19070pLenti PGK GFP Puro (w509-5)3rd gen lentiviral eGFP expression vector, PGK promoter, Puro 
Loading...
19135pENTR4-V5-LUC (w468-1)Gateway entry vector, V5-Luciferase
Loading...
19166pLenti PGK Blast V5-LUC (w528-1)3rd gen lentiviral V5-Luciferase expression vector, PGK promoter, Blast
Loading...
19167pQCXIN X2/pTER shEGFP#1 (w491-1)Retroviral eGFP shRNA #2 (H1/TO promoter), 3’LTR insertion, Neo
Loading...
19170pENTR/pSM2(CMV) GFP (w513-1)Gateway entry vector for miRNA expression under CMV promoter and GFP reporter
Loading...
19360pLenti PGK V5-LUC Puro (w543-1)3rd gen lentiviral V5-Luciferase expression vector, PGK promoter, Puro
Loading...
19364pENTR1A-GFP-N2 (FR1)Gateway entry vector for C-term eGFP fusion, frame N2
Loading...
19732pLenti CMV GFP DEST (736-1)3rd gen lentiviral Gateway destination vector, expression/RNAi, eGFP co-expression
Loading...
19785pLenti CMV/TO V5-LUC Puro (w549-1)3rd gen lentiviral V5-Luciferase expression vector, CMV/TO promoter, Puro
Loading...
20025pENTR4-HA-myc-nuc (w557-1)Gateway entry vector for nuclear targeting of domains (N-term HA , C-term NLS, myc tags)
Loading...
20026pENTR4-myc-nuc (w556-1)Gateway entry vector for nuclear targeting of domains (C-term NLS, myc tags)
Loading...