|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||19364||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2348
Vector typeGateway Entry vector
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameEnhanced Green Fluorescent Protein
Insert Size (bp)719
- Cloning method Restriction Enzyme
- 5′ cloning site Bam HI (not destroyed)
- 3′ cloning site Not I (not destroyed)
- 5′ sequencing primer ENTRforw
- 3′ sequencing primer ENTRrev (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byGFP derived from the pEGFP-N1 plasmid (Clontech).
Terms and Licenses
- Not Available to Industry
Articles Citing this Plasmid
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pENTR1A-GFP-N2 (FR1) was a gift from Eric Campeau & Paul Kaufman (Addgene plasmid # 19364 ; http://n2t.net/addgene:19364 ; RRID:Addgene_19364)
For your References section:A versatile viral system for expression and depletion of proteins in mammalian cells. Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, Campisi J, Yaswen P, Cooper PK, Kaufman PD.. PLoS One. 2009 Aug 6;4(8):e6529. 10.1371/journal.pone.0006529 PubMed 19657394