pLenti CMV GFP DEST (736-1)
(Plasmid #19732)

• Purpose: (Empty Backbone) 3rd gen lentiviral Gateway destination vector, expression/RNAi, eGFP co-expression
• Depositing Labs: Eric Campeau, Paul Kaufman
• Publication: Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.
• Citations: 11 Articles

Backbone

• Vector backbone: p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
• Backbone size (bp): 9205
• Vector type: Mammalian Expression, Lentiviral, RNAi, Gateway Cloning

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DB3.1
• Growth instructions: DB3.1, 370C
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: n/a

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• A portion of this plasmid was derived from a plasmid made by: GFP cDNA derived from pEGFP-C1 (Clontech).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There are minor discrepancies between Addgene's quality control sequence and the reference sequence provided by the depositing lab. These discrepancies should not affect plasmid function.