pLenti X1 Puro DEST (694-6)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17297||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
|Cloning Grade DNA||17297-DNA.cg||2 µg of cloning grade DNA in Tris buffer||1||$95|
This material is available to academics and nonprofits only.
Vector backbonep156RRL-sinPPT-CMV-GFP-PRE/Nhe I
- Backbone size (bp) 9014
Vector typeMammalian Expression, Lentiviral, RNAi ; Destination vector
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Ampicillin
Growth instructionsDB3.1 or ccDB survival, 37oC
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Please note that there are several mismatches in sequence between depositor's sequence and Addgene's quality control sequence for this plasmid. These mismatches are in backbone regions and should not affect function.
Information for Cloning Grade DNA (Catalog # 17297-DNA.cg) ( Back to top )
Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.
- Amount 2 µg
- Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
- Pricing $95 USD
- Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).
Terms and Licenses
- Not Available to Industry
Addgene has verified this plasmid using Next Generation Sequencing. Results are available here
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLenti X1 Puro DEST (694-6) was a gift from Eric Campeau & Paul Kaufman (Addgene plasmid # 17297 ; http://n2t.net/addgene:17297 ; RRID:Addgene_17297)
For your References section:A versatile viral system for expression and depletion of proteins in mammalian cells. Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, Campisi J, Yaswen P, Cooper PK, Kaufman PD.. PLoS One. 2009 Aug 6;4(8):e6529. 10.1371/journal.pone.0006529 PubMed 19657394