Addgene - pLenti CMV Hygro DEST (w117-1) Plasmid Data

Plasmid 17454: pLenti CMV Hygro DEST (w117-1)

Purpose:
3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Hygro
Gene/insert name:
None
Vector backbone:
p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral ; Destination vector
Backbone size (bp):
10319
5' sequencing primer:
CMVforw List of Sequencing Primers
Bacterial resistance(s)
Ampicillin and Chloramphenicol
Growth strain(s)
DB3.1
Growth temperature (℃):
37
Growth instructions:
DB3.1 or ccDB survival, 37oC
High or low copy:
High Copy
Selectable markers:
Hygromycin
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Eric Campeau
Terms and Licenses:
MTA

Comments:

Please note that there are several mismatches between depositor's sequence and Addgene's quality control sequence. These mismatches are in the backbone regions only and do not affect any of the features.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A versatile viral system for expression and depletion of proteins in mammalian cells. Campeau et al (PLoS One. 2009 Aug 6;4(8):e6529. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17454" in your Materials and Methods section.