pLenti CMV Hygro DEST (w117-1)
(Plasmid #17454)

• Purpose: (Empty Backbone) 3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Hygro
• Depositing Labs: Eric Campeau, Paul Kaufman
• Publication: Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Backbone

• Vector backbone: p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
  (Search Vector Database)
• Backbone size (bp): 10319
• Vector type: Mammalian Expression, Lentiviral ; Destination vector
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin
• Growth Temperature: 37°C
• Growth Strain(s): DB3.1
• Growth instructions: DB3.1 or ccDB survival, 37oC
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CMVforw

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• Terms and Licenses:
  • UBMTA
  • Ancillary Agreement for Plasmids Containing FP Materials
  • Institut Pasteur Label License for cPPT
• Industry Terms:
  • Not Available to Industry
• Articles Citing this Plasmid:
  • 23 References

Depositor Comments

Please note that there are several mismatches between depositor's sequence and Addgene's quality control sequence. These mismatches are in the backbone regions only and do not affect any of the features.

Information for Cloning Grade DNA (Catalog # 17454-DNA.cg) ( Back to top )

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

• Amount: 2 µg
• Guaranteed Concentration: 100 ng/µl +/- 5 ng/µl
• Pricing: $95 USD
• Storage: DNA can be stored at 4℃ (short term) or -20℃ (long term).

Resource Information

• Terms and Licenses:
  • UBMTA
  • Ancillary Agreement for Plasmids Containing FP Materials
  • Institut Pasteur Label License for cPPT
• Industry Terms:
  • Not Available to Industry

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here