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Purpose(Empty Backbone) 3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Hygro
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Depositing Lab
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Publication
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Price$65 (USD)
Add to Cart
Available to Academic and Nonprofits Only
Backbone
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Vector backbonep156RRL-sinPPT-CMV-GFP-PRE/Nhe I
- Backbone size (bp) 10319
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Vector typeMammalian Expression, Lentiviral ; Destination vector
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin and Chloramphenicol
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Growth Temperature37°C
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Growth Strain(s)DB3.1
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Growth instructionsDB3.1 or ccDB survival, 37oC
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Copy numberHigh Copy
Sequence Information
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Depositor Sequences
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Addgene Sequences
Gene/Insert
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Gene/Insert nameNone
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMVforw (Common Sequencing Primers)
Resource Information
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Terms and Licenses
Depositor Comments
Please note that there are several mismatches between depositor's sequence and Addgene's quality control sequence. These mismatches are in the backbone regions only and do not affect any of the features.
How to cite this plasmid
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti CMV Hygro DEST (w117-1) was a gift from Eric Campeau (Addgene plasmid # 17454) -
For your References section:
A versatile viral system for expression and depletion of proteins in mammalian cells. Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, Campisi J, Yaswen P, Cooper PK, Kaufman PD.. PLoS One. 2009 Aug 6;4(8):e6529. 10.1371/journal.pone.0006529 PubMed 19657394
Map generated by Addgene from full sequence supplied by depositor.
Map uploaded by the depositor.
