pLenti CMV Hygro DEST (w117-1)
(Plasmid #17454)

• Purpose: (Empty Backbone) 3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Hygro
• Depositing Labs: Eric Campeau, Paul Kaufman
• Publication: Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.
• Sequence Information:

  • Depositor Sequences: None.
  • Addgene Sequences: Full (1) Partial (1)

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	17454	Plasmid sent as bacteria in agar stab	1	$65	Add to Cart

This material is available to academics and nonprofits only.

Backbone

• Vector backbone: p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
  (Search Vector Database)
• Backbone size (bp): 10319
• Vector type: Mammalian Expression, Lentiviral ; Destination vector
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin
• Growth Temperature: 37°C
• Growth Strain(s): DB3.1
• Growth instructions: DB3.1 or ccDB survival, 37oC
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CMVforw

Resource Information

• Terms and Licenses:
  • UBMTA
  • Ancillary Agreement for Plasmids Containing FP Materials
• Articles Citing this Plasmid:
  • 6 References

Depositor Comments

Please note that there are several mismatches between depositor's sequence and Addgene's quality control sequence. These mismatches are in the backbone regions only and do not affect any of the features.