Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene

pLenti CMV Hygro DEST (w117-1) Citations (36)

Originally described in: A versatile viral system for expression and depletion of proteins in mammalian cells.
Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, Campisi J, Yaswen P, Cooper PK, Kaufman PD. PLoS One. 2009 Aug 6;4(8):e6529.
PubMed Journal

Articles Citing pLenti CMV Hygro DEST (w117-1)

Articles
miR-301a as an NF-kappaB activator in pancreatic cancer cells. Lu Z, Li Y, Takwi A, Li B, Zhang J, Conklin DJ, Young KH, Martin R, Li Y. EMBO J. 2011 Jan 5;30(1):57-67. doi: 10.1038/emboj.2010.296. Epub 2010 Nov 26. PubMed
UV-sensitive syndrome protein UVSSA recruits USP7 to regulate transcription-coupled repair. Schwertman P, Lagarou A, Dekkers DH, Raams A, van der Hoek AC, Laffeber C, Hoeijmakers JH, Demmers JA, Fousteri M, Vermeulen W, Marteijn JA. Nat Genet. 2012 May;44(5):598-602. doi: 10.1038/ng.2230. PubMed
Human herpesvirus 6B immediate-early I protein contains functional HLA-A*02, HLA-A*03, and HLA-B*07 class I restricted CD8(+) T-cell epitopes. Iampietro M, Morissette G, Gravel A, Dubuc I, Rousseau M, Hasan A, O'Reilly RJ, Flamand L. Eur J Immunol. 2014 Dec;44(12):3573-84. doi: 10.1002/eji.201444931. Epub 2014 Oct 29. PubMed
Loss of TRIM33 causes resistance to BET bromodomain inhibitors through MYC- and TGF-beta-dependent mechanisms. Shi X, Mihaylova VT, Kuruvilla L, Chen F, Viviano S, Baldassarre M, Sperandio D, Martinez R, Yue P, Bates JG, Breckenridge DG, Schlessinger J, Turk BE, Calderwood DA. Proc Natl Acad Sci U S A. 2016 Aug 2;113(31):E4558-66. doi: 10.1073/pnas.1608319113. Epub 2016 Jul 18. PubMed
Dual-color Herpesvirus Capsids Discriminate Inoculum from Progeny and Reveal Axonal Transport Dynamics. Scherer J, Yaffe ZA, Vershinin M, Enquist LW. J Virol. 2016 Aug 31. pii: JVI.01122-16. PubMed
PROTOCADHERIN 7 Acts through SET and PP2A to Potentiate MAPK Signaling by EGFR and KRAS during Lung Tumorigenesis. Zhou X, Updegraff BL, Guo Y, Peyton M, Girard L, Larsen JE, Xie XJ, Zhou Y, Hwang TH, Xie Y, Rodriguez-Canales J, Villalobos P, Behrens C, Wistuba II, Minna JD, O'Donnell KA. Cancer Res. 2017 Jan 1;77(1):187-197. doi: 10.1158/0008-5472.CAN-16-1267-T. Epub 2016 Nov 7. PubMed

Associated Plasmids

Phosphatidylinositol-(4, 5)-bisphosphate regulates calcium gating of small-conductance cation channel TMEM16F. Ye W, Han TW, Nassar LM, Zubia M, Jan YN, Jan LY. Proc Natl Acad Sci U S A. 2018 Feb 13;115(7):E1667-E1674. doi: 10.1073/pnas.1718728115. Epub 2018 Jan 30. PubMed
HDAC11 suppresses the thermogenic program of adipose tissue via BRD2. Bagchi RA, Ferguson BS, Stratton MS, Hu T, Cavasin MA, Sun L, Lin YH, Liu D, Londono P, Song K, Pino MF, Sparks LM, Smith SR, Scherer PE, Collins S, Seto E, McKinsey TA. JCI Insight. 2018 Aug 9;3(15). pii: 120159. doi: 10.1172/jci.insight.120159. eCollection 2018 Aug 9. PubMed
Modulation of Mutant Kras(G12D) -Driven Lung Tumorigenesis In Vivo by Gain or Loss of PCDH7 Function. Zhou X, Padanad MS, Evers BM, Smith B, Novaresi N, Suresh S, Richardson JA, Stein E, Zhu J, Hammer RE, O'Donnell KA. Mol Cancer Res. 2019 Feb;17(2):594-603. doi: 10.1158/1541-7786.MCR-18-0739. Epub 2018 Nov 8. PubMed
Chemically induced vesiculation as a platform for studying TMEM16F activity. Han TW, Ye W, Bethel NP, Zubia M, Kim A, Li KH, Burlingame AL, Grabe M, Jan YN, Jan LY. Proc Natl Acad Sci U S A. 2019 Jan 22;116(4):1309-1318. doi: 10.1073/pnas.1817498116. Epub 2019 Jan 8. PubMed
FACT subunit Spt16 controls UVSSA recruitment to lesion-stalled RNA Pol II and stimulates TC-NER. Wienholz F, Zhou D, Turkyilmaz Y, Schwertman P, Tresini M, Pines A, van Toorn M, Bezstarosti K, Demmers JAA, Marteijn JA. Nucleic Acids Res. 2019 May 7;47(8):4011-4025. doi: 10.1093/nar/gkz055. PubMed
CATACOMB: An endogenous inducible gene that antagonizes H3K27 methylation activity of Polycomb repressive complex 2 via an H3K27M-like mechanism. Piunti A, Smith ER, Morgan MAJ, Ugarenko M, Khaltyan N, Helmin KA, Ryan CA, Murray DC, Rickels RA, Yilmaz BD, Rendleman EJ, Savas JN, Singer BD, Bulun SE, Shilatifard A. Sci Adv. 2019 Jul 3;5(7):eaax2887. doi: 10.1126/sciadv.aax2887. eCollection 2019 Jul. PubMed
Dynamic change of electrostatic field in TMEM16F permeation pathway shifts its ion selectivity. Ye W, Han TW, He M, Jan YN, Jan LY. Elife. 2019 Jul 18;8. pii: 45187. doi: 10.7554/eLife.45187. PubMed
Modelling the MYC-driven normal-to-tumour switch in breast cancer. Lourenco C, Kalkat M, Houlahan KE, De Melo J, Longo J, Done SJ, Boutros PC, Penn LZ. Dis Model Mech. 2019 Jul 26;12(7). pii: 12/7/dmm038083. doi: 10.1242/dmm.038083. PubMed
CLIC4 and CLIC1 bridge plasma membrane and cortical actin network for a successful cytokinesis. Uretmen Kagiali ZC, Saner N, Akdag M, Sanal E, Degirmenci BS, Mollaoglu G, Ozlu N. Life Sci Alliance. 2019 Dec 26;3(2). pii: 3/2/e201900558. doi: 10.26508/lsa.201900558. Print 2020 Feb. PubMed
Integrin alpha5 promotes migration and cisplatin resistance in esophageal squamous cell carcinoma cells. Hou S, Jin W, Xiao W, Deng B, Wu D, Zhi J, Wu K, Cao X, Chen S, Ding Y, Shi H. Am J Cancer Res. 2019 Dec 1;9(12):2774-2788. eCollection 2019. PubMed
AXL and CAV-1 play a role for MTH1 inhibitor TH1579 sensitivity in cutaneous malignant melanoma. Das I, Gad H, Brautigam L, Pudelko L, Tuominen R, Hoiom V, Almlof I, Rajagopal V, Hansson J, Helleday T, Egyhazi Brage S, Warpman Berglund U. Cell Death Differ. 2020 Jul;27(7):2081-2098. doi: 10.1038/s41418-019-0488-1. Epub 2020 Jan 9. PubMed
Serine phosphorylation of the small phosphoprotein ICAP1 inhibits its nuclear accumulation. Su VL, Simon B, Draheim KM, Calderwood DA. J Biol Chem. 2020 Mar 6;295(10):3269-3284. doi: 10.1074/jbc.RA119.009794. Epub 2020 Jan 31. PubMed
Proximal Biotinylation-Based Combinatory Approach for Isolating Integral Plasma Membrane Proteins. Akdag M, Yunt ZS, Kamacioglu A, Qureshi MH, Akarlar BA, Ozlu N. J Proteome Res. 2020 Aug 7;19(8):3583-3592. doi: 10.1021/acs.jproteome.0c00113. Epub 2020 Jun 15. PubMed
A simple protein-based surrogate neutralization assay for SARS-CoV-2. Abe KT, Li Z, Samson R, Samavarchi-Tehrani P, Valcourt EJ, Wood H, Budylowski P, Dupuis AP 2nd, Girardin RC, Rathod B, Wang JH, Barrios-Rodiles M, Colwill K, McGeer AJ, Mubareka S, Gommerman JL, Durocher Y, Ostrowski M, McDonough KA, Drebot MA, Drews SJ, Rini JM, Gingras AC. JCI Insight. 2020 Oct 2;5(19). pii: 142362. doi: 10.1172/jci.insight.142362. PubMed
Multivalent tumor suppressor adenomatous polyposis coli promotes Axin biomolecular condensate formation and efficient beta-catenin degradation. Li TM, Ren J, Husmann D, Coan JP, Gozani O, Chua KF. Sci Rep. 2020 Oct 15;10(1):17425. doi: 10.1038/s41598-020-74080-2. PubMed
A versatile oblique plane microscope for large-scale and high-resolution imaging of subcellular dynamics. Sapoznik E, Chang BJ, Huh J, Ju RJ, Azarova EV, Pohlkamp T, Welf ES, Broadbent D, Carisey AF, Stehbens SJ, Lee KM, Marin A, Hanker AB, Schmidt JC, Arteaga CL, Yang B, Kobayashi Y, Tata PR, Kruithoff R, Doubrovinski K, Shepherd DP, Millett-Sikking A, York AG, Dean KM, Fiolka RP. Elife. 2020 Nov 12;9. pii: 57681. doi: 10.7554/eLife.57681. PubMed
Amplification-free detection of SARS-CoV-2 with CRISPR-Cas13a and mobile phone microscopy. Fozouni P, Son S, Diaz de Leon Derby M, Knott GJ, Gray CN, D'Ambrosio MV, Zhao C, Switz NA, Kumar GR, Stephens SI, Boehm D, Tsou CL, Shu J, Bhuiya A, Armstrong M, Harris AR, Chen PY, Osterloh JM, Meyer-Franke A, Joehnk B, Walcott K, Sil A, Langelier C, Pollard KS, Crawford ED, Puschnik AS, Phelps M, Kistler A, DeRisi JL, Doudna JA, Fletcher DA, Ott M. Cell. 2021 Jan 21;184(2):323-333.e9. doi: 10.1016/j.cell.2020.12.001. Epub 2020 Dec 4. PubMed
Genetic Screens Identify Host Factors for SARS-CoV-2 and Common Cold Coronaviruses. Wang R, Simoneau CR, Kulsuptrakul J, Bouhaddou M, Travisano KA, Hayashi JM, Carlson-Stevermer J, Zengel JR, Richards CM, Fozouni P, Oki J, Rodriguez L, Joehnk B, Walcott K, Holden K, Sil A, Carette JE, Krogan NJ, Ott M, Puschnik AS. Cell. 2021 Jan 7;184(1):106-119.e14. doi: 10.1016/j.cell.2020.12.004. Epub 2020 Dec 9. PubMed
Rapid 3D phenotypic analysis of neurons and organoids using data-driven cell segmentation-free machine learning. Mergenthaler P, Hariharan S, Pemberton JM, Lourenco C, Penn LZ, Andrews DW. PLoS Comput Biol. 2021 Feb 22;17(2):e1008630. doi: 10.1371/journal.pcbi.1008630. eCollection 2021 Feb. PubMed
BRAF Modulates Stretch-Induced Intercellular Gap Formation through Localized Actin Reorganization. Hollosi A, Paszty K, Kellermayer M, Charras G, Varga A. Int J Mol Sci. 2021 Aug 20;22(16). pii: ijms22168989. doi: 10.3390/ijms22168989. PubMed
NSD2 dimethylation at H3K36 promotes lung adenocarcinoma pathogenesis. Sengupta D, Zeng L, Li Y, Hausmann S, Ghosh D, Yuan G, Nguyen TN, Lyu R, Caporicci M, Morales Benitez A, Coles GL, Kharchenko V, Czaban I, Azhibek D, Fischle W, Jaremko M, Wistuba II, Sage J, Jaremko L, Li W, Mazur PK, Gozani O. Mol Cell. 2021 Nov 4;81(21):4481-4492.e9. doi: 10.1016/j.molcel.2021.08.034. Epub 2021 Sep 22. PubMed
Development and Initial Characterization of Cellular Models for COG Complex-Related CDG-II Diseases. Sumya FT, Pokrovskaya ID, Lupashin V. Front Genet. 2021 Sep 17;12:733048. doi: 10.3389/fgene.2021.733048. eCollection 2021. PubMed
Subtractive CRISPR screen identifies the ATG16L1/vacuolar ATPase axis as required for non-canonical LC3 lipidation. Ulferts R, Marcassa E, Timimi L, Lee LC, Daley A, Montaner B, Turner SD, Florey O, Baillie JK, Beale R. Cell Rep. 2021 Oct 26;37(4):109899. doi: 10.1016/j.celrep.2021.109899. PubMed
SOX10 Regulates Melanoma Immunogenicity through an IRF4-IRF1 Axis. Yokoyama S, Takahashi A, Kikuchi R, Nishibu S, Lo JA, Hejna M, Moon WM, Kato S, Zhou Y, Hodi FS, Song JS, Sakurai H, Fisher DE, Hayakawa Y. Cancer Res. 2021 Dec 15;81(24):6131-6141. doi: 10.1158/0008-5472.CAN-21-2078. Epub 2021 Nov 2. PubMed
SARS-CoV-2 Virus-Like Particle Neutralizing Capacity in Blood Donors Depends on Serological Profile and Donor-Declared SARS-CoV-2 Vaccination History. Drews SJ, Hu Q, Samson R, Abe KT, Rathod B, Colwill K, Gingras AC, Yi QL, O'Brien SF. Microbiol Spectr. 2022 Feb 23;10(1):e0226221. doi: 10.1128/spectrum.02262-21. Epub 2022 Feb 16. PubMed
KSR induces RAS-independent MAPK pathway activation and modulates the efficacy of KRAS inhibitors. Paniagua G, Jacob HKC, Brehey O, Garcia-Alonso S, Lechuga CG, Pons T, Musteanu M, Guerra C, Drosten M, Barbacid M. Mol Oncol. 2022 Sep;16(17):3066-3081. doi: 10.1002/1878-0261.13213. Epub 2022 Apr 12. PubMed
Ribosome stalling during selenoprotein translation exposes a ferroptosis vulnerability. Li Z, Ferguson L, Deol KK, Roberts MA, Magtanong L, Hendricks JM, Mousa GA, Kilinc S, Schaefer K, Wells JA, Bassik MC, Goga A, Dixon SJ, Ingolia NT, Olzmann JA. Nat Chem Biol. 2022 Jul;18(7):751-761. doi: 10.1038/s41589-022-01033-3. Epub 2022 May 30. PubMed
Relating individual cell division events to single-cell ERK and Akt activity time courses. Stern AD, Smith GR, Santos LC, Sarmah D, Zhang X, Lu X, Iuricich F, Pandey G, Iyengar R, Birtwistle MR. Sci Rep. 2022 Oct 27;12(1):18077. doi: 10.1038/s41598-022-23071-6. PubMed
The HAPSTR2 retrogene buffers stress signaling and resilience in mammals. Amici DR, Cingoz H, Alasady MJ, Alhayek S, Phoumyvong CM, Sahni N, Yi SS, Mendillo ML. Nat Commun. 2023 Jan 11;14(1):152. doi: 10.1038/s41467-022-35697-1. PubMed
Mitochondrial redox adaptations enable alternative aspartate synthesis in SDH-deficient cells. Hart ML, Quon E, Vigil ABG, Engstrom IA, Newsom OJ, Davidsen K, Hoellerbauer P, Carlisle SM, Sullivan LB. Elife. 2023 Mar 8;12:e78654. doi: 10.7554/eLife.78654. PubMed

If you have published an article using this material, please email us at [email protected] to have your article added to this page.