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An engineered biosensor enables dynamic aspartate measurements in living cells.
Davidsen K, Marvin JS, Aggarwal A, Brown TA, Sullivan LB
Elife. 2024 Feb 23;12:RP90024. doi: 10.7554/eLife.90024. PubMed Article

Plasmids from Article

ID Plasmid Purpose
203458pRSET-jAspSnFR3jAspSnFR3 in T7 bacterial expression vector
203459pAAV-CAG-jAspSnFR3-mRuby3jAspSnFR3-mRuby3 in adenoviral expression vector
203460pENTR1a-jAspSnFR3jAspSnFR3 in Gateway cloning entry vector
203461pENTR1a-MTS-jAspSnFR3Mitochondrial directed jAspSnFR3 in Gateway cloning entry vector
203487pLenti-MTS-jAspSnFR3Mitochondrial directed jAspSnFR3 in lentiviral expression vector
203488pLenti-jAspSnFR3jAspSnFR3 in lentiviral expression vector
203489pENTR1a-jAspSnFR3-mRuby3-NLSNuclear directed jAspSnFR3-mRuby3 in Gateway cloning entry vector
203490pENTR1a-MTS-jAspSnFR3-mRuby3Mitochondrial directed jAspSnFR3-mRuby3 in Gateway cloning entry vector
203491pENTR1a-jAspSnFR3-mRuby3jAspSnFR3-mRuby3 in Gateway cloning entry vector
203492pLenti-MTS-jAspSnFR3-mRuby3Mitochondrial directed jAspSnFR3-mRuby3 in lentiviral expression vector
203494pLenti-jAspSnFR3-mRuby3-NLSNuclear directed jAspSnFR3-mRuby3 in lentiviral expression vector
203498pLenti-jAspSnFR3-mRuby3jAspSnFR3-mRuby3 in lentiviral expression vector
203500pLX304-CAG-jAspSnFR3-mRuby3jAspSnFR3-mRuby3 in lentiviral expression vector

Antibodies from Article