pET28a Halotag-CNA35
(Plasmid #131128)

• Purpose: Halotag-CNA35 peptide for Collagen labelling
• Depositing Lab: Samantha Stehbens
• Publication: Ju et al bioRxiv 2022

Backbone

• Vector backbone: pET28a
• Total vector size (bp): 7181
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH5alpha
• Growth instructions: Use BL21 (DE3) for protein expression.
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Halotag-CNA35
• Alt name: Halotag-Collagen-binding adhesion protein 35
• Species: staphylococcus aureus
• Insert Size (bp): 1929
• Tag / Fusion Protein:
  • Halotag (N terminal on backbone)

Resource Information

• Supplemental Documents:
  • pET28a_HaloTag_CNA35.gb
• A portion of this plasmid was derived from a plasmid made by: https://www.addgene.org/61603/

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • HaloTag Vector Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Modified from https://www.addgene.org/61603/ to express Halotag-CNA35.

Please visit https://www.biorxiv.org/content/10.1101/2022.02.08.479516v2 for bioRxiv preprint.