pLenti CMV GFP Puro (658-5)
(Plasmid #17448)

• Purpose: 3rd gen lentiviral eGFP expression vector, CMV promoter, Puro
• Depositing Labs: Eric Campeau, Paul Kaufman
• Publication: Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.
• Citations: 226 Articles

Backbone

• Vector backbone: p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
• Backbone size w/o insert (bp): 7889
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Stbl3, 37oC
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GFP
• Alt name: enhanced GFP
• Insert Size (bp): 719

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: Sal I (not destroyed)
• 5′ sequencing primer: CMVforw

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The 3'LTR of this plasmid has a TT (or AA on the sense strand) in the sequence. This sequence is consistent with other available LTR sequences and should not affect plasmid function.