PurposeExpresses PP7 coat protein fused to mCherry
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||61763||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backboneLentiviral vector
- Backbone size w/o insert (bp) 4997
- Total vector size (bp) 7549
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namePP7 coat protein fused to mCherry
Alt namePP7 coat protein
SpeciesPsuedomonas Phage PP7
Insert Size (bp)1107
- Promoter Ubc promoter
/ Fusion Protein
- mCherry (C terminal on insert)
- Cloning method Gateway Cloning
- 5′ sequencing primer CCGGGCCCGCTCTAGATTGAAT (Common Sequencing Primers)
Note: Addgene's quality control sequencing finds an S136Y amino acid residue substitution in mCherry. The depositing laboratory is confident that the gene product is fully functional and has used it routinely.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:PP7_mCherry was a gift from Daniel Larson (Addgene plasmid # 61763 ; http://n2t.net/addgene:61763 ; RRID:Addgene_61763)
For your References section:Kinetic competition during the transcription cycle results in stochastic RNA processing. Coulon A, Ferguson ML, de Turris V, Palangat M, Chow CC, Larson DR. Elife. 2014 Oct 1;3. doi: 10.7554/eLife.03939. 10.7554/eLife.03939 PubMed 25271374