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pMS2-GFP
(Plasmid #27121)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 27121 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MS2
  • Species
    bacteriophage MS2
  • Tags / Fusion Proteins
    • HA (N terminal on insert)
    • EGFP (C terminal on insert)
    • SV40 NLS (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer M13_Rev
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Under the control of the promoter of the large subunit of RNA polymerase II

NB: There is a 14aa gap in the MS2 sequence starting at aa#67 when compared with GenBank ID: NP_040648.1

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMS2-GFP was a gift from Robert Singer (Addgene plasmid # 27121 ; http://n2t.net/addgene:27121 ; RRID:Addgene_27121)
  • For your References section:

    Single mRNA molecules demonstrate probabilistic movement in living mammalian cells. Fusco D, Accornero N, Lavoie B, Shenoy SM, Blanchard JM, Singer RH, Bertrand E. Curr Biol. 2003 Jan 21. 13(2):161-7. 10.1016/S0960-9822(02)01436-7 PubMed 12546792