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PurposeHuman expression plasmid for SaCas9 KKH variant: CAG-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
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| Plasmid | 70708 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCAG-CFP
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Backbone manufacturerCepko lab (Addgene plasmid# 11179)
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemammalian codon-optimized KKH variant Staphylococcus aureus Cas9
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Alt nameSauCas9
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SpeciesS. aureus
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MutationE782K, N968K and R1015H in SaCas9
- Promoter CAG
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Tag
/ Fusion Protein
- NLS-3xFLAG (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer oBK280 (5'-CCTACAGCTCCTGGGCAACG-3') (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MSP1830 was a gift from Keith Joung (Addgene plasmid # 70708 ; http://n2t.net/addgene:70708 ; RRID:Addgene_70708) -
For your References section:
Broadening the targeting range of Staphylococcus aureus CRISPR-Cas9 by modifying PAM recognition. Kleinstiver BP, Prew MS, Tsai SQ, Nguyen NT, Topkar VV, Zheng Z, Joung JK. Nat Biotechnol. 2015 Nov 2. doi: 10.1038/nbt.3404. 10.1038/nbt.3404 PubMed 26524662
Map uploaded by the depositor.
