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(Plasmid #70708)


Item Catalog # Description Quantity Price (USD)
Plasmid 70708 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Cepko lab (Addgene plasmid# 11179)
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    mammalian codon-optimized KKH variant Staphylococcus aureus Cas9(E782K/N968K/R1015H)-NLS-3xFlag
  • Species
    S. aureus
  • Mutation
    E782K, N968K and R1015H in SaCas9
  • Promoter CAG
  • Tags / Fusion Proteins
    • NLS (C terminal on insert)
    • 3x FLAG (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MSP1830 was a gift from Keith Joung (Addgene plasmid # 70708 ; ; RRID:Addgene_70708)
  • For your References section:

    Broadening the targeting range of Staphylococcus aureus CRISPR-Cas9 by modifying PAM recognition. Kleinstiver BP, Prew MS, Tsai SQ, Nguyen NT, Topkar VV, Zheng Z, Joung JK. Nat Biotechnol. 2015 Nov 2. doi: 10.1038/nbt.3404. 10.1038/nbt.3404 PubMed 26524662