PurposeHuman expression vector for S .aureus Cas9: CAG-humanSaCas9-NLS-3xFLAG
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||65776||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, CRISPR
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)XL1 Blue
Copy numberHigh Copy
Gene/Insert namemammalian codon-optimized Staphylococcus aureus Cas9-NLS-3xFlag
- Promoter CAG
/ Fusion Proteins
- NLS (C terminal on insert)
- 3x FLAG (C terminal on insert)
- Cloning method Gibson Cloning
- 5′ sequencing primer oBK280 (5'-CCTACAGCTCCTGGGCAACG-3') (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:BPK2139 was a gift from Keith Joung (Addgene plasmid # 65776 ; http://n2t.net/addgene:65776 ; RRID:Addgene_65776)
For your References section:Engineered CRISPR-Cas9 nucleases with altered PAM specificities. Kleinstiver BP, Prew MS, Tsai SQ, Topkar VV, Nguyen NT, Zheng Z, Gonzales AP, Li Z, Peterson RT, Yeh JJ, Aryee MJ, Joung JK. Nature. 2015 Jun 22. doi: 10.1038/nature14592. 10.1038/nature14592 PubMed 26098369