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pEGFPN1-human Dynamin 1aa K44A
(Plasmid #22197)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 22197 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression, Bacterial Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human Dynamin 1aa K44A
  • Alt name
    dyn1 K44A
  • Alt name
    DNM1 K44A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2595
  • Mutation
    K44A
  • GenBank ID
    AAA02803.1
  • Entrez Gene
    DNM1 (a.k.a. DEE31, DNM, EIEE31)
  • Tag / Fusion Protein
    • GFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII/ BamHI (destroyed during cloning)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer EGFP-N Sequencing Primer
  • 3′ sequencing primer EGFP-C Sequencing Primer
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFPN1-human Dynamin 1aa K44A was a gift from Pietro De Camilli (Addgene plasmid # 22197 ; http://n2t.net/addgene:22197 ; RRID:Addgene_22197)
  • For your References section:

    Dynamin at actin tails. Lee E, De Camilli P. Proc Natl Acad Sci U S A. 2002 Jan 8. 99(1):161-6. 10.1073/pnas.012607799 PubMed 11782545