A CRISPR Dropout Screen Identifies Genetic Vulnerabilities and Therapeutic Targets in Acute Myeloid Leukemia.
Tzelepis K, Koike-Yusa H, De Braekeleer E, Li Y, Metzakopian E, Dovey OM, Mupo A, Grinkevich V, Li M, Mazan M, Gozdecka M, Ohnishi S, Cooper J, Patel M, McKerrell T, Chen B, Domingues AF, Gallipoli P, Teichmann S, Ponstingl H, McDermott U, Saez-Rodriguez J, Huntly BJ, Iorio F, Pina C, Vassiliou GS, Yusa K
Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 10.1016/j.celrep.2016.09.079. PubMed Article
Tzelepis K, Koike-Yusa H, De Braekeleer E, Li Y, Metzakopian E, Dovey OM, Mupo A, Grinkevich V, Li M, Mazan M, Gozdecka M, Ohnishi S, Cooper J, Patel M, McKerrell T, Chen B, Domingues AF, Gallipoli P, Teichmann S, Ponstingl H, McDermott U, Saez-Rodriguez J, Huntly BJ, Iorio F, Pina C, Vassiliou GS, Yusa K
Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 10.1016/j.celrep.2016.09.079. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 67974 | pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W | CRISPR gRNA expression vector with an improved scaffold and puro/BFP markers |
| 67975 | pKLV2-U6gRNA5(BbsI)-PGKpuro2AZsG-W | CRISPR gRNA expression vector with an improved scaffold and puro/ZsG markers |
| 67976 | pKLV2-U6gRNA5(BbsI)-PGKpuro2AmAG-W | CRISPR gRNA expression vector with an improved scaffold and puro/mAG markers |
| 67977 | pKLV2-U6gRNA5(BbsI)-PGKpuro2AmCherry-W | CRISPR gRNA expression vector with an improved scaffold and puro/mCherry markers |
| 67978 | pKLV2-EF1a-BsdCas9-W | Lentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the N-terminus |
| 67979 | pKLV2-U6gRNA5(Empty)-PGKBFP2AGFP-W | Cas9 activity reporter (control) with BFP and GFP. |
| 67980 | pKLV2-U6gRNA5(gGFP)-PGKBFP2AGFP-W | Cas9 activity reporter with BFP and GFP. |
| 67981 | pKLV2-U6gRNA5(Empty)-PGKmCherry2AGFP-W | Cas9 activity reporter (control) with mCherry and GFP |
| 67982 | pKLV2-U6gRNA5(gGFP)-PGKmCherry2AGFP-W | Cas9 activity reporter with mCherry and GFP |
| 67983 | pKLV2-U6gRNA5(Empty)-PGKGFP2ABFP-W | Cas9 activity reporter (control) with GFP and BFP |
| 67984 | pKLV2-U6gRNA5(gBFP)-PGKGFP2ABFP-W | Cas9 activity reporter with GFP and BFP |
| 67985 | pKLV2-U6gRNA5(Empty)-PGKmCherry2ABFP-W | Cas9 activity reporter (control) with mCherry and BFP |
| 67986 | pKLV2-U6gRNA5(gBFP)-PGKmCherry2ABFP-W | Cas9 activity reporter with mCherry and BFP |
| 67987 | pRosa26-EF1a-hCas9IRESneo | Mouse Rosa26 targeting vector carrying the EF1a-hCas9-IRES-neo cassette |
| 67988 | Mouse genome-wide lentiviral CRISPR gRNA library version 2 | Mouse genome-wide CRISPR guide RNA library V2. (pKLV2-U6gRNA5(MouseV2)-PGKpuro2ABFP-W) |
| 67989 | Human genome-wide lentiviral CRISPR gRNA library version 1 | Human genome-wide CRISPR guide RNA library V1 (pKLV2-U6gRNA5(HumanV1)-PGKpuro2ABFP-W) |
| 67990 | pKLV2-U6gRNA3(BbsI)-PGKpuro2ABFP | CRISPR gRNA expression vector with the conventional scaffold and puro/BFP markers without WPRE |
| 67991 | pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP | CRISPR gRNA expression vector with an improved l scaffold and puro/BFP markers without WPRE |
| 68343 | pKLV2-EF1a-Cas9Bsd-W | Lentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the C-terminus |
| 72666 | pKLV2.2-h7SKgRNA5(SapI)-hU6gRNA5(BbsI)-PGKpuroBFP-W | Lentiviral dual CRISPR gRNA expression vector (human 7SK and human U6 promoters) |
| 72667 | pKLV2.2-mU6gRNA5(SapI)-hU6gRNA5(BbsI)-PGKpuroBFP-W | Lentiviral dual CRISPR gRNA expression vector (mouse U6 and human U6 promoters) |