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A CRISPR Dropout Screen Identifies Genetic Vulnerabilities and Therapeutic Targets in Acute Myeloid Leukemia.
Tzelepis K, Koike-Yusa H, De Braekeleer E, Li Y, Metzakopian E, Dovey OM, Mupo A, Grinkevich V, Li M, Mazan M, Gozdecka M, Ohnishi S, Cooper J, Patel M, McKerrell T, Chen B, Domingues AF, Gallipoli P, Teichmann S, Ponstingl H, McDermott U, Saez-Rodriguez J, Huntly BJ, Iorio F, Pina C, Vassiliou GS, Yusa K
Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 10.1016/j.celrep.2016.09.079.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
67974pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-WCRISPR gRNA expression vector with an improved scaffold and puro/BFP markers
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67975pKLV2-U6gRNA5(BbsI)-PGKpuro2AZsG-WCRISPR gRNA expression vector with an improved scaffold and puro/ZsG markers
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67976pKLV2-U6gRNA5(BbsI)-PGKpuro2AmAG-WCRISPR gRNA expression vector with an improved scaffold and puro/mAG markers
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67977pKLV2-U6gRNA5(BbsI)-PGKpuro2AmCherry-WCRISPR gRNA expression vector with an improved scaffold and puro/mCherry markers
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67978pKLV2-EF1a-BsdCas9-WLentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the N-terminus
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67979pKLV2-U6gRNA5(Empty)-PGKBFP2AGFP-WCas9 activity reporter (control) with BFP and GFP.
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67980pKLV2-U6gRNA5(gGFP)-PGKBFP2AGFP-WCas9 activity reporter with BFP and GFP.
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67981pKLV2-U6gRNA5(Empty)-PGKmCherry2AGFP-WCas9 activity reporter (control) with mCherry and GFP
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67982pKLV2-U6gRNA5(gGFP)-PGKmCherry2AGFP-WCas9 activity reporter with mCherry and GFP
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67983pKLV2-U6gRNA5(Empty)-PGKGFP2ABFP-WCas9 activity reporter (control) with GFP and BFP
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67984pKLV2-U6gRNA5(gBFP)-PGKGFP2ABFP-WCas9 activity reporter with GFP and BFP
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67985pKLV2-U6gRNA5(Empty)-PGKmCherry2ABFP-WCas9 activity reporter (control) with mCherry and BFP
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67986pKLV2-U6gRNA5(gBFP)-PGKmCherry2ABFP-WCas9 activity reporter with mCherry and BFP
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67987pRosa26-EF1a-hCas9IRESneoMouse Rosa26 targeting vector carrying the EF1a-hCas9-IRES-neo cassette
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67988Mouse genome-wide lentiviral CRISPR gRNA library version 2Mouse genome-wide CRISPR guide RNA library V2. (pKLV2-U6gRNA5(MouseV2)-PGKpuro2ABFP-W)
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67989Human genome-wide lentiviral CRISPR gRNA library version 1Human genome-wide CRISPR guide RNA library V1 (pKLV2-U6gRNA5(HumanV1)-PGKpuro2ABFP-W)
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67990pKLV2-U6gRNA3(BbsI)-PGKpuro2ABFPCRISPR gRNA expression vector with the conventional scaffold and puro/BFP markers without WPRE
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67991pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFPCRISPR gRNA expression vector with an improved l scaffold and puro/BFP markers without WPRE
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68343pKLV2-EF1a-Cas9Bsd-WLentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the C-terminus
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72666pKLV2.2-h7SKgRNA5(SapI)-hU6gRNA5(BbsI)-PGKpuroBFP-WLentiviral dual CRISPR gRNA expression vector (human 7SK and human U6 promoters)
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72667pKLV2.2-mU6gRNA5(SapI)-hU6gRNA5(BbsI)-PGKpuroBFP-WLentiviral dual CRISPR gRNA expression vector (mouse U6 and human U6 promoters)
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