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pcDNA3-myc3-bTrCP
(Plasmid #20718)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 20718 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3-myc3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5486
  • Vector type
    Mammalian Expression
  • Selectable markers
    G418

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    beta-transducin repeat containing (BTRC)
  • Alt name
    BETA-TRCP, FBW1A, FBXW1, FBXW1A, FWD1, MGC4643, bTrCP, bTrCP1, betaTrCP
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1710
  • Mutation
    *
  • GenBank ID
    NG_009234 AF099932
  • Tag / Fusion Protein
    • Myc3 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Data not available (not destroyed)
  • 3′ cloning site Data not available (destroyed during cloning)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

*possible Y377I mutation in stock. This mutation is not known to affect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-myc3-bTrCP was a gift from Yue Xiong (Addgene plasmid # 20718 ; http://n2t.net/addgene:20718 ; RRID:Addgene_20718)
  • For your References section:

    Phosphorylation- and Skp1-independent in vitro ubiquitination of E2F1 by multiple ROC-cullin ligases. Ohta T, Xiong Y. Cancer Res. 2001 Feb 15. 61(4):1347-53. PubMed 11245432