Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


(Plasmid #20718)


This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20718 Standard format: Plasmid sent in bacteria as agar stab 1 $85


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5486
  • Vector type
    Mammalian Expression
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy


  • Gene/Insert name
    beta-transducin repeat containing (BTRC)
  • Alt name
    BETA-TRCP, FBW1A, FBXW1, FBXW1A, FWD1, MGC4643, bTrCP, bTrCP1, betaTrCP
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
  • Mutation
  • GenBank ID
    NG_009234 AF099932
  • Entrez Gene
    Btrc (a.k.a. Beta-Trcp1, E3RS-IkappaB, E3RSIkappaB, FWD1, Fbw1a, HOS, SCF b-TRCP, Slimb, b-TrCP, beta-TrCP)
  • Tag / Fusion Protein
    • Myc3 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Data not available (not destroyed)
  • 3′ cloning site Data not available (destroyed during cloning)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

*possible Y377I mutation in stock. This mutation is not known to affect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-myc3-bTrCP was a gift from Yue Xiong (Addgene plasmid # 20718 ; ; RRID:Addgene_20718)
  • For your References section:

    Phosphorylation- and Skp1-independent in vitro ubiquitination of E2F1 by multiple ROC-cullin ligases. Ohta T, Xiong Y. Cancer Res. 2001 Feb 15. 61(4):1347-53. PubMed 11245432