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pcDNA3-myc-Skp2
(Plasmid #19947)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 19947 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5437
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    S-phase kinase-associated protein 2 (p45)
  • Alt name
    Skp2
  • Alt name
    p45
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1305
  • GenBank ID
  • Entrez Gene
    SKP2 (a.k.a. FBL1, FBXL1, FLB1, p45)
  • Tag / Fusion Protein
    • myc (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Please note that the 436 amino acid Skp2 insert in this plasmid does not exactly match GenBank reference sequences for Skp2. The Skp2 insert was PCR amplified from a HeLa cell library and appears to be a combination of the first 12 amino acids of reference sequence AAC50242.1 (containing a H2D mutation due to the primer used for cloning) followed by amino acids 1-424 of NP_005974.2, with a F343I mutation (numbering according to 436aa Skp2).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-myc-Skp2 was a gift from Yue Xiong (Addgene plasmid # 19947 ; http://n2t.net/addgene:19947 ; RRID:Addgene_19947)
  • For your References section:

    Phosphorylation- and Skp1-independent in vitro ubiquitination of E2F1 by multiple ROC-cullin ligases. Ohta T, Xiong Y. Cancer Res. 2001 Feb 15. 61(4):1347-53. PubMed 11245432