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p4489 Flag-betaTrCP
(Plasmid #10865)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 10865 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5446
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    betaTrCP
  • Species
    H. sapiens (human)
  • Entrez Gene
    BTRC (a.k.a. BETA-TRCP, FBW1A, FBXW1, FBXW1A, FWD1, bTrCP, bTrCP1, betaTrCP)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p4489 Flag-betaTrCP was a gift from Peter Howley (Addgene plasmid # 10865 ; http://n2t.net/addgene:10865 ; RRID:Addgene_10865)
  • For your References section:

    Harnessing the ubiquitination machinery to target the degradation of specific cellular proteins. Zhou P, Bogacki R, McReynolds L, Howley PM. Mol Cell. 2000 Sep . 6(3):751-6. 10.1016/S1097-2765(00)00074-5 PubMed 11030355