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Purpose(Empty Backbone) C-terminal fusion to moxGFP
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 68070 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepEGFP-N1
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Backbone manufacturerClontech
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Modifications to backboneoxFP inserted via AgeI/NotI restriction sites, removing EGFP
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Vector typeMammalian Expression
- Promoter CMV
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Tag
/ Fusion Protein
- moxGFP (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F
- 3′ sequencing primer EGFP-N (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
moxGFP was a gift from Erik Snapp (Addgene plasmid # 68070 ; http://n2t.net/addgene:68070 ; RRID:Addgene_68070) -
For your References section:
A palette of fluorescent proteins optimized for diverse cellular environments. Costantini LM, Baloban M, Markwardt ML, Rizzo M, Guo F, Verkhusha VV, Snapp EL. Nat Commun. 2015 Jul 9;6:7670. doi: 10.1038/ncomms8670. 10.1038/ncomms8670 PubMed 26158227
