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Tracking genome engineering outcome at individual DNA breakpoints.
Certo MT, Ryu BY, Annis JE, Garibov M, Jarjour J, Rawlings DJ, Scharenberg AM
Nat Methods. 2011 Jul 10;8(8):671-6. doi: 10.1038/nmeth.1648.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
31475pCVL SFFV d14GFP Donor
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31476pCVL SFFV d14GFP EF1s HA.NLS.Sce(opt)
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31477pCVL SFFV HA.NLS.I-AniIY2(reo).T2A.TagBFP
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31478pCVL SFFV HA.NLS.I-AniIY2K227M(reo).T2A.TagBFP
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31479pCVL SFFV-EF1s HA.NLS.Sce(opt)
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31480pCVL Traffic Light Reporter 1.1 (Ani target) Ef1a Puro
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31481pCVL Traffic Light Reporter 1.1 (Sce target) Ef1a BFP
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31482pCVL Traffic Light Reporter 1.1 (Sce target) Ef1a Puro
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31483pCVL Traffic Light Reporter 2.1 (VF2468 ZFN target) Ef1a
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31484pRRL sEF1a HA.NLS.Sce(opt).T2A.IFP
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31485pRRL SFFV d20GFP.T2A.mTagBFP Donor
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63586psPAX2-D64VpsPAX2-D64V is derived from psPAX2 plasmid. It is a packaging plasmid for making Integrase deficient lentiviral vectors since has a point mutation in the integrase gene.
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