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pCVL SFFV HA.NLS.I-AniIY2K227M(reo).T2A.TagBFP
(Plasmid #31478)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 31478 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SFFV HA NLS Y2 K227M T2A BFP
  • Insert Size (bp)
    2010
  • Mutation
    F13Y, S111Y, K227M = Y2 nickase "reo" (restriction endonuclease optimized) denotes that the coding sequence has had silent restriction sites placed into it to aid in enzyme redesign.
  • Tags / Fusion Proteins
    • HA
    • NLS

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCVL SFFV HA.NLS.I-AniIY2K227M(reo).T2A.TagBFP was a gift from Andrew Scharenberg (Addgene plasmid # 31478 ; http://n2t.net/addgene:31478 ; RRID:Addgene_31478)
  • For your References section:

    Tracking genome engineering outcome at individual DNA breakpoints. Certo MT, Ryu BY, Annis JE, Garibov M, Jarjour J, Rawlings DJ, Scharenberg AM. Nat Methods. 2011 Jul 10;8(8):671-6. doi: 10.1038/nmeth.1648. 10.1038/nmeth.1648 PubMed 21743461