pCVL SFFV HA.NLS.I-AniIY2K227M(reo).T2A.TagBFP
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||31478||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5000
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Gene/Insert nameSFFV HA NLS Y2 K227M T2A BFP
Insert Size (bp)2010
MutationF13Y, S111Y, K227M = Y2 nickase "reo" (restriction endonuclease optimized) denotes that the coding sequence has had silent restriction sites placed into it to aid in enzyme redesign.
/ Fusion Proteins
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCVL SFFV HA.NLS.I-AniIY2K227M(reo).T2A.TagBFP was a gift from Andrew Scharenberg (Addgene plasmid # 31478 ; http://n2t.net/addgene:31478 ; RRID:Addgene_31478)
For your References section:Tracking genome engineering outcome at individual DNA breakpoints. Certo MT, Ryu BY, Annis JE, Garibov M, Jarjour J, Rawlings DJ, Scharenberg AM. Nat Methods. 2011 Jul 10;8(8):671-6. doi: 10.1038/nmeth.1648. 10.1038/nmeth.1648 PubMed 21743461