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pRRL SFFV d20GFP.T2A.mTagBFP Donor
(Plasmid #31485)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 31485 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRRL
  • Backbone size w/o insert (bp) 6200
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SFFV d20BFP T2A BFP
  • Insert Size (bp)
    1861
  • Mutation
    GFP has 20 amino acids deleted from the C terminus.

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRRL SFFV d20GFP.T2A.mTagBFP Donor was a gift from Andrew Scharenberg (Addgene plasmid # 31485 ; http://n2t.net/addgene:31485 ; RRID:Addgene_31485)
  • For your References section:

    Tracking genome engineering outcome at individual DNA breakpoints. Certo MT, Ryu BY, Annis JE, Garibov M, Jarjour J, Rawlings DJ, Scharenberg AM. Nat Methods. 2011 Jul 10;8(8):671-6. doi: 10.1038/nmeth.1648. 10.1038/nmeth.1648 PubMed 21743461