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pCVL Traffic Light Reporter 1.1 (Ani target) Ef1a Puro
(Plasmid #31480)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 31480 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCVL
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Traffic Light Reporter 1.1 (ani target)
  • Insert Size (bp)
    2803
  • Mutation
    mCherry has M9S, M16L to reduce background mCherry fluorescence from internal start codons. This is what ".1 indicates"

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCVL Traffic Light Reporter 1.1 (Ani target) Ef1a Puro was a gift from Andrew Scharenberg (Addgene plasmid # 31480 ; http://n2t.net/addgene:31480 ; RRID:Addgene_31480)
  • For your References section:

    Tracking genome engineering outcome at individual DNA breakpoints. Certo MT, Ryu BY, Annis JE, Garibov M, Jarjour J, Rawlings DJ, Scharenberg AM. Nat Methods. 2011 Jul 10;8(8):671-6. doi: 10.1038/nmeth.1648. 10.1038/nmeth.1648 PubMed 21743461