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pCVL Traffic Light Reporter 2.1 (VF2468 ZFN target) Ef1a
(Plasmid #31483)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 31483 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCVL
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Traffic Light Reporter 2.1 (V2468 zfn target) EF Puro
  • Insert Size (bp)
    2939
  • Mutation
    Traffic Light 2.0 series have a +3 PEST sequence that aids in degradation of the +3 "gibberishFP" produced by mutNHEJ events. The mCherry sequence has M9S and M16L mutations that reduce background fluorescence from internal start codons. This is what ".1" stands for.

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCVL Traffic Light Reporter 2.1 (VF2468 ZFN target) Ef1a was a gift from Andrew Scharenberg (Addgene plasmid # 31483 ; http://n2t.net/addgene:31483 ; RRID:Addgene_31483)
  • For your References section:

    Tracking genome engineering outcome at individual DNA breakpoints. Certo MT, Ryu BY, Annis JE, Garibov M, Jarjour J, Rawlings DJ, Scharenberg AM. Nat Methods. 2011 Jul 10;8(8):671-6. doi: 10.1038/nmeth.1648. 10.1038/nmeth.1648 PubMed 21743461