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(Plasmid #41645)


Item Catalog # Description Quantity Price (USD)
Plasmid 41645 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 5370
  • Total vector size (bp) 6801
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
    XL10 Gold
  • Copy number
    High Copy


  • Gene/Insert name
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
  • Mutation
    K145N on Dronpa (allows control of photochromic FP domain association by light)
  • GenBank ID
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site Xho1 (not destroyed)
  • 5′ sequencing primer XpressFWD: TATGGCTAGCATGACTGGT
  • 3′ sequencing primer SP6: ATTTAGGTGACACTATAG
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-mNeptune2-N was a gift from Michael Lin (Addgene plasmid # 41645 ; ; RRID:Addgene_41645)
  • For your References section:

    Optical control of protein activity by fluorescent protein domains. Zhou XX, Chung HK, Lam AJ, Lin MZ. Science. 2012 Nov 9;338(6108):810-4. doi: 10.1126/science.1226854. 10.1126/science.1226854 PubMed 23139335