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Purpose
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Plasmid | 41645 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepcDNA3
- Backbone size w/o insert (bp) 5370
- Total vector size (bp) 6801
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)XL10 Gold
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemNeptune2-Dronpa145N
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SpeciesH. sapiens (human), Synthetic
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Insert Size (bp)1431
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MutationK145N on Dronpa (allows control of photochromic FP domain association by light)
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GenBank ID
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site Xho1 (not destroyed)
- 5′ sequencing primer XpressFWD: TATGGCTAGCATGACTGGT
- 3′ sequencing primer SP6: ATTTAGGTGACACTATAG (Common Sequencing Primers)
Resource Information
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Terms and Licenses
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Industry Terms
- Not Available to Industry
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Articles Citing this Plasmid
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-mNeptune2-N was a gift from Michael Lin (Addgene plasmid # 41645 ; http://n2t.net/addgene:41645 ; RRID:Addgene_41645) -
For your References section:
Optical control of protein activity by fluorescent protein domains. Zhou XX, Chung HK, Lam AJ, Lin MZ. Science. 2012 Nov 9;338(6108):810-4. doi: 10.1126/science.1226854. 10.1126/science.1226854 PubMed 23139335