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Erik Snapp Lab Plasmids


The Erik Snapp Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

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Title Authors Publication
BiP availability distinguishes states of homeostasis and stress in the endoplasmic reticulum of living cells. Lai CW, Aronson DE, Snapp EL Mol Biol Cell. 2010 Jun 15;21(12):1909-21. doi: 10.1091/mbc.E09-12-1066. Epub 2010 Apr 21.
Monitoring chaperone engagement of substrates in the endoplasmic reticulum of live cells. Snapp EL, Sharma A, Lippincott-Schwartz J, Hegde RS Proc Natl Acad Sci U S A. 2006 Apr 25;103(17):6536-41. Epub 2006 Apr 14.
Assessing the tendency of fluorescent proteins to oligomerize under physiologic conditions. Costantini LM, Fossati M, Francolini M, Snapp EL Traffic. 2012 May;13(5):643-9. doi: 10.1111/j.1600-0854.2012.01336.x. Epub 2012 Feb 20.
Cysteineless non-glycosylated monomeric blue fluorescent protein, secBFP2, for studies in the eukaryotic secretory pathway. Costantini LM, Subach OM, Jaureguiberry-bravo M, Verkhusha VV, Snapp EL Biochem Biophys Res Commun. 2013 Jan 18;430(3):1114-9. doi: 10.1016/j.bbrc.2012.12.028. Epub 2012 Dec 19.
A palette of fluorescent proteins optimized for diverse cellular environments. Costantini LM, Baloban M, Markwardt ML, Rizzo M, Guo F, Verkhusha VV, Snapp EL Nat Commun. 2015 Jul 9;6:7670. doi: 10.1038/ncomms8670.
moxDendra2: an inert photoswitchable protein for oxidizing environments. Kaberniuk AA, Morano NC, Verkhusha VV, Snapp EL Chem Commun (Camb). 2017 Feb 9;53(13):2106-2109. doi: 10.1039/c6cc09997a.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
62230psecBFP2secBFP2 (Synthetic)Mammalian ExpressionCysteineless non-glycosylated monomeric blue fluorescent protein, secBFP2, for studies in the eukaryotic secretory pathway. Biochem Biophys Res Commun. 2013 Jan 18;430(3):1114-9. doi: 10.1016/j.bbrc.2012.12.028. Epub 2012 Dec 19. encodes an inert blue fluorescent protein for use in oxidizing cellular environments including the periplasm and mammalian secretory pathway. clontech N1 Add to Cart
62231BiP-mGFPhamster BiP-mGFP-KDEL (Synthetic)Mammalian ExpressionBiP availability distinguishes states of homeostasis and stress in the endoplasmic reticulum of living cells. Mol Biol Cell. 2010 Jun 15;21(12):1909-21. doi: 10.1091/mbc.E09-12-1066. Epub 2010 Apr 21. Encodes hamster BiP/GRP78/HSPA5 fused to monomeric EGFP. Useful for studying BiP mobility and trafficking in live cells. clontech N1 Add to Cart
62232BiP-mGFP P495Lhamster BiP-mGFP-KDEL (Synthetic)Mammalian ExpressionBiP availability distinguishes states of homeostasis and stress in the endoplasmic reticulum of living cells. Mol Biol Cell. 2010 Jun 15;21(12):1909-21. doi: 10.1091/mbc.E09-12-1066. Epub 2010 Apr 21. Mutant of hamster BiP-mGFP unable to bind clients. clontech N1 Add to Cart
62233BiP-mCherryhamster BiP-mCherry-KDEL (Synthetic)Mammalian ExpressionBiP availability distinguishes states of homeostasis and stress in the endoplasmic reticulum of living cells. Mol Biol Cell. 2010 Jun 15;21(12):1909-21. doi: 10.1091/mbc.E09-12-1066. Epub 2010 Apr 21. Hamster BiP/GRP78/HSPA5 fused to mCherry. Useful for studying BiP mobility and trafficking in live cells. clontech N1 Add to Cart
62236ER-mRFPER-mRFP (Synthetic)Mammalian ExpressionMonitoring chaperone engagement of substrates in the endoplasmic reticulum of live cells. Proc Natl Acad Sci U S A. 2006 Apr 25;103(17):6536-41. Epub 2006 Apr 14. Plasmid expresses endoplasmic reticulum targeted mRFP in mammalian cells. clontech C1 Add to Cart
62237CyTERM-mGFPCyTERM-mGFP (Synthetic)Mammalian ExpressionAssessing the tendency of fluorescent proteins to oligomerize under physiologic conditions. Traffic. 2012 May;13(5):643-9. doi: 10.1111/j.1600-0854.2012.01336.x. Epub 2012 Feb 20. ER targeted transmembrane domain with monomeric EGFP oriented towards the cytoplasm. Used for assessing fluorescent protein oligomerization. clontech N1 Add to Cart
68063oxBFPMammalian ExpressionA palette of fluorescent proteins optimized for diverse cellular environments. Nat Commun. 2015 Jul 9;6:7670. doi: 10.1038/ncomms8670. C-terminal fusion to oxBFP pEGFP-N1
  • Tag / Fusion Protein
    • oxBFP (C terminal on backbone)
  • Add to Cart
    68064moxBFPMammalian ExpressionA palette of fluorescent proteins optimized for diverse cellular environments. Nat Commun. 2015 Jul 9;6:7670. doi: 10.1038/ncomms8670. C-terminal fusion to oxBFP pEGFP-N1
  • Tag / Fusion Protein
    • moxBFP (C terminal on backbone)
  • Add to Cart
    68066moxCerulean3Mammalian ExpressionA palette of fluorescent proteins optimized for diverse cellular environments. Nat Commun. 2015 Jul 9;6:7670. doi: 10.1038/ncomms8670. C-terminal fusion to moxCerulean3 pEGFP-N1
  • Tag / Fusion Protein
    • moxCerulean3 (C terminal on backbone)
  • Add to Cart
    68067oxVenusMammalian ExpressionA palette of fluorescent proteins optimized for diverse cellular environments. Nat Commun. 2015 Jul 9;6:7670. doi: 10.1038/ncomms8670. C-terminal fusion to oxVenus pEGFP-N1
  • Tag / Fusion Protein
    • oxVenus (C terminal on backbone)
  • Add to Cart
    68068moxVenusMammalian ExpressionA palette of fluorescent proteins optimized for diverse cellular environments. Nat Commun. 2015 Jul 9;6:7670. doi: 10.1038/ncomms8670. C-terminal fusion to moxVenus pEGFP-N1
  • Tag / Fusion Protein
    • moxVenus (C terminal on backbone)
  • Add to Cart
    68069oxGFPMammalian ExpressionA palette of fluorescent proteins optimized for diverse cellular environments. Nat Commun. 2015 Jul 9;6:7670. doi: 10.1038/ncomms8670. C-terminal fusion to oxGFP pEGFP-N1
  • Tag / Fusion Protein
    • oxGFP (C terminal on backbone)
  • Add to Cart
    68070moxGFPMammalian ExpressionA palette of fluorescent proteins optimized for diverse cellular environments. Nat Commun. 2015 Jul 9;6:7670. doi: 10.1038/ncomms8670. C-terminal fusion to moxGFP pEGFP-N1
  • Tag / Fusion Protein
    • moxGFP (C terminal on backbone)
  • Add to Cart
    68072ERmoxGFPmoxGFP (Synthetic)Mammalian ExpressionA palette of fluorescent proteins optimized for diverse cellular environments. Nat Commun. 2015 Jul 9;6:7670. doi: 10.1038/ncomms8670. mammalian expression of ER localized moxGFP ER-mRFP Add to Cart
    68073GalToxBFPGalT signal anchor transmembrane domain (Other)Mammalian ExpressionA palette of fluorescent proteins optimized for diverse cellular environments. Nat Commun. 2015 Jul 9;6:7670. doi: 10.1038/ncomms8670. mammalian expression of GC localized moxGFP oxBFP Add to Cart
    68126ERoxBFPoxBFP (Synthetic)Mammalian ExpressionA palette of fluorescent proteins optimized for diverse cellular environments. Nat Commun. 2015 Jul 9;6:7670. doi: 10.1038/ncomms8670. mammalian expression of ER localized oxBFP ER-mRFP Add to Cart
    89789GalT-moxDendra2GalT signal anchor transmembrane domain (Homo sapiens)Mammalian ExpressionmoxDendra2: an inert photoswitchable protein for oxidizing environments. Chem Commun (Camb). 2017 Feb 9;53(13):2106-2109. doi: 10.1039/c6cc09997a. mammalian expression of GC localized moxDendra2 N1-moxDendra2 Add to Cart
    89791pUC57 moxDendra2moxDendra2 (Synthetic)cloning vectormoxDendra2: an inert photoswitchable protein for oxidizing environments. Chem Commun (Camb). 2017 Feb 9;53(13):2106-2109. doi: 10.1039/c6cc09997a. bacterial expression of moxDendra2 pUC57 Add to Cart
    89792N1-moxDendra2Mammalian ExpressionmoxDendra2: an inert photoswitchable protein for oxidizing environments. Chem Commun (Camb). 2017 Feb 9;53(13):2106-2109. doi: 10.1039/c6cc09997a. C-terminal fusion to moxDendra2 pEGFP-N1
  • Tag / Fusion Protein
    • moxDendra2 (C terminal on backbone)
  • Add to Cart