288,339 results

1. pCW-Cas9

   Plasmid

   #50661

   Purpose

   Doxycycline-inducible lentiviral expression of SpCas9

   Depositor

   Eric Lander, David Sabatini

   Article

   Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

   Insert

   humanized S. pyogenes Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Tet ON

   Available Since

   Jan. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNIC28-DENV3-NS5FL

   Plasmid

   #86013

   Purpose

   Expression of dengue virus 3 NS5 protein

   Depositor

   Dahai Luo

   Article

   Zhao et al PLoS Pathog. 2015 Mar 16;11(3):e1004682.

   Insert

   DENV3-NS5FL
   

   Use

   Tags

   hexa-histidine tag

   Expression

   Bacterial

   Mutation

   amino acid residues 6 to 895 of DENV3 NS5

   Promoter

   T7

   Available Since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNIC28-DENV3-NS5FL

   Plasmid

   #86013

   Purpose

   Expression of dengue virus 3 NS5 protein

   Depositor

   Dahai Luo

   Article

   Zhao et al PLoS Pathog. 2015 Mar 16;11(3):e1004682.

   Insert

   DENV3-NS5FL
   

   Use

   Tags

   hexa-histidine tag

   Expression

   Bacterial

   Mutation

   amino acid residues 6 to 895 of DENV3 NS5

   Promoter

   T7

   Available Since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLAMP1-mCherry

   Plasmid

   #45147

   Depositor

   Amy Palmer

   Article

   Van Engelenburg et al Nat Methods. 2010 Apr;7(4):325-30. doi:

   Insert

   LAMP1-mCherry (LAMP1 Human, Synthetic)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLAMP1-mCherry

   Plasmid

   #45147

   Depositor

   Amy Palmer

   Article

   Van Engelenburg et al Nat Methods. 2010 Apr;7(4):325-30. doi:

   Insert

   LAMP1-mCherry (LAMP1 Human, Synthetic)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIRE4-Bpa

   Plasmid

   #155342

   Purpose

   Plasmid encoding the translational pair that enables efficient incorporation of Benzoyl-phenylalanine in response to the amber codon into proteins in mammmalian cells.

   Depositor

   Irene Coin

   Article

   Bottke et al EMBO Rep. 2020 Sep 14:e50437. doi: 10.15

   Insert

   humanized EBpaRS/BstYam
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK/U6

   Available Since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIRE4-Bpa

   Plasmid

   #155342

   Purpose

   Plasmid encoding the translational pair that enables efficient incorporation of Benzoyl-phenylalanine in response to the amber codon into proteins in mammmalian cells.

   Depositor

   Irene Coin

   Article

   Bottke et al EMBO Rep. 2020 Sep 14:e50437. doi: 10.15

   Insert

   humanized EBpaRS/BstYam
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK/U6

   Available Since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRGEB32

   Plasmid

   #63142

   Purpose

   Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for Agrobacterium-mediated transformation.

   Depositor

   Yinong Yang

   Article

   Xie et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Rice snoRNA U3 promoter for gRNA/PTG expression a…

   Available Since

   April 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRGEB32

   Plasmid

   #63142

   Purpose

   Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for Agrobacterium-mediated transformation.

   Depositor

   Yinong Yang

   Article

   Xie et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Rice snoRNA U3 promoter for gRNA/PTG expression a…

   Available Since

   April 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40 (AAV Retrograde)

    Viral Prep

    #105551-AAVrg

    Purpose

    Ready-to-use AAV Retrograde particles produced from pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40 (#105551). In addition to the viral particles, you will also receive purified pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40 plasmid DNA. Expression of GFP-Cre from CamKII promoter. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    James M. Wilson

    Promoter

    CaMKII

    Tags

    GFP

    Available Since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

11. pCMVR8.74

    Plasmid

    #22036

    Purpose

    2nd generation lentiviral packaging plasmid. Can be used with 2nd or 3rd generation lentiviral vectors and envelope expressing plasmid (Addgene#12259)

    Depositor

    Didier Trono

    Insert

    gag pol tat rev
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMVR8.74

    Plasmid

    #22036

    Purpose

    2nd generation lentiviral packaging plasmid. Can be used with 2nd or 3rd generation lentiviral vectors and envelope expressing plasmid (Addgene#12259)

    Depositor

    Didier Trono

    Insert

    gag pol tat rev
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-CaMKIIa-EGFP (AAV5)

    Viral Prep

    #50469-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV-CaMKIIa-EGFP (#50469). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-EGFP plasmid DNA. CamKIIa-driven EGFP expression control. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Bryan Roth

    Promoter

    CaMKII

    Tags

    EGFP

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

14. sgRNA(MS2) cloning backbone

    Plasmid

    #61424

    Purpose

    sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Dec. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. sgRNA(MS2) cloning backbone

    Plasmid

    #61424

    Purpose

    sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Dec. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV_ABEmax_P2A_GFP

    Plasmid

    #112101

    Purpose

    A:T-to-G:C base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

    Insert

    ABEmax_P2A_GFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV_ABEmax_P2A_GFP

    Plasmid

    #112101

    Purpose

    A:T-to-G:C base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

    Insert

    ABEmax_P2A_GFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST (AAV9)

    Viral Prep

    #174007-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST (#174007). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST plasmid DNA. Syn-driven, Cre-dependent expression of calcium sensor GCaMP8s and soma-targeted ChrimsonR. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Mark Histed

    Article

    LaFosse et al eNeuro. 2023 Mar 29;10(3):ENEURO.0378-22

    Promoter

    Syn

    Tags

    None

    Available Since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

19. pUBC-SV40NLS-Pfv-Sapphire

    Plasmid

    #203653

    Purpose

    Mammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC promoter.

    Depositor

    Liam Holt

    Article

    Shu T et et al bioRxiv 2022

    Insert

    PfV
    

    Use

    Lentiviral

    Tags

    Sapphire

    Expression

    Mutation

    Promoter

    Available Since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. EGFP-CAAX

    Plasmid

    #86056

    Purpose

    A mammalian expression plasmid encoding farnesylation motif of HRAS with N-terminus EGFP.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    Homo sapiens Harvey rat sarcoma (HRAS Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    contains the region from 825-857 of HRAS as a far…

    Promoter

    CMV

    Available Since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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