168,234 results

1. pMXs-Klf4

   Plasmid

   #13370

   Purpose

   Retroviral expression of mouse Klf4

   Depositor

   Shinya Yamanaka

   Article

   Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

   Insert

   Kruppel-like factor 4 (gut) (Klf4 Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   None

   Available Since

   Jan. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP-hsRPA32 (MSW#497)

   Plasmid

   #208068

   Purpose

   Expression of GFP-tagged hsRPA2 in human cells

   Depositor

   Marc Wold

   Article

   Haring et al J Biol Chem. 2008 Jul 4;283(27):19095-11

   Insert

   RPA2 (RPA2 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   T88I- please see depositor comments

   Available Since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSBtet-Hyg

   Plasmid

   #60508

   Purpose

   SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of rtTA and hygromycin resistance gene

   Depositor

   Eric Kowarz

   Article

   Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

   Type

   Empty backbone

   Use

   Transposon

   Expression

   Mammalian

   Promoter

   TCE & RPBSA

   Available Since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. p6599 MSCV-IP N-HAonly FOSL1

   Plasmid

   #34897

   Depositor

   Peter Howley

   Article

   White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

   Insert

   FOSL1 (FOSL1 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   MSCV LTR

   Available Since

   Feb. 8, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSANG10-3F-BG4

   Plasmid

   #55756

   Purpose

   Expresses BG4 scFv anti-G-quadruplex antibody in bacteria

   Depositor

   Shankar Balasubramanian

   Article

   Biffi et al Nat Chem. 2013 Mar;5(3):182-6. doi: 10.1

   Insert

   BG4
   

   Tags

   6xHis, pelB leader, and tri-FLAG

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. p667-UBC-NEK7-V5-miniTurbo_IDG-K

   Plasmid

   #189882

   Purpose

   Gateway destination clone of NEK7 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   NEK7 (NEK7 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5-miniTurbo

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLX307-VHL-3F-BirA

   Plasmid

   #220147

   Purpose

   Expresses VHL-BirA for VHL substrate identification by E-STUB

   Depositor

   William Sellers

   Article

   Huang et al Nat Chem Biol. 2024 Mar 21. doi: 10.1038

   Insert

   von Hippel-Lindau tumor suppressor (VHL Human)
   

   Use

   Lentiviral

   Tags

   3F-BirA

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   EF-1a

   Available Since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. SpyCatcher003-sfGFP

   Plasmid

   #133449

   Purpose

   Expresses SpyCatcher003-superfolder GFP in bacterial cytoplasm

   Depositor

   Mark Howarth

   Article

   Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

   Insert

   SpyCatcher003-sfGFP
   

   Tags

   His6 and TEV site

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hSyn-DIO-HA-hM3D(Gq)-IRES-mCitrine (AAV8)

   Viral Prep

   #50454-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-hSyn-DIO-HA-hM3D(Gq)-IRES-mCitrine (#50454). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-HA-hM3D(Gq)-IRES-mCitrine plasmid DNA. Synapsin-driven, Cre-dependent, excitatory hM3D DREADD expression, with physically separate mCitrine expression for cell body labeling. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Bryan Roth

   Promoter

   Syn

   Tags

   mCitrine (Cre-dependent)

   Available Since

   Sept. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

10. pMXs-Sox2

    Plasmid

    #13367

    Purpose

    Retroviral expression of mouse Sox2

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

    Insert

    SRY-box containing gene 2 (Sox2 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAGGS_3E5E_eGFP

    Plasmid

    #103054

    Purpose

    Encodes Ebola virus minigenome with EGFP reporter gene. Minigenome is under control of the CAG promoter

    Depositor

    Elke Mühlberger

    Article

    Nelson et al Antiviral Res. 2017 Aug 12;146:21-27. do

    Insert

    Ebola virus (ebolavirus Zaire, Mayinga) minigenome
    

    Tags

    3E5E eGFP cloned into pCAGGS vector from pCDNA3.1…

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDESTmycYBX1

    Plasmid

    #19878

    Depositor

    Thomas Tuschl

    Article

    Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

    Insert

    Y box binding protein 1 (YBX1 Human)
    

    Tags

    MYC

    Expression

    Mammalian

    Available Since

    Dec. 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. attB-puro donor.dna

    Plasmid

    #181923

    Purpose

    attB-puro DNA donor for twinPE and Bxb1 mediated gene-size insertion

    Depositor

    David Liu

    Article

    Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

    Insert

    attB-EGFP-EF1α-PuroR-T2A-TagBFP
    

    Expression

    Mammalian

    Available Since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMXs-Oct3/4

    Plasmid

    #13366

    Purpose

    Retroviral expression of mouse Oct3/4

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

    Insert

    POU domain, class 5, transcription factor 1 (Pou5f1 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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15. MoClo Plant Parts Kit

    Plasmid Kit

    #1000000047

    Purpose

    Modular Cloning (MoClo) collection contains 95 standardized parts to enable Golden Gate construction of multi-gene constructs for plant transformation

    Depositor

    Sylvestre Marillonnet, Nicola Patron

    Available Since

    April 8, 2014

    Availability

    Academic Institutions and Nonprofits only

16. CaMKII-somBiPOLES-mCerulean (AAV9)

    Viral Prep

    #154948-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from CaMKII-somBiPOLES-mCerulean (#154948). In addition to the viral particles, you will also receive purified CaMKII-somBiPOLES-mCerulean plasmid DNA. CamKII-driven expression of soma-targeted BiPOLES for optogenetic inhibition (blue light) and activation (red light). These AAV preparations are suitable purity for injection into animals.

    Depositor

    Simon Wiegert

    Article

    Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

    Promoter

    CaMKIIa(0.4)

    Tags

    mCerulean

    Available Since

    Sept. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

17. pAAV-hSyn-EGFP (AAV5 trial size)

    Viral Prep

    #50465-AAV5.T

    Purpose

    Ready-to-use AAV5 trial size particles produced from pAAV-hSyn-EGFP (#50465). In addition to the viral particles, you will also receive purified pAAV-hSyn-EGFP plasmid DNA. hSyn-driven EGFP expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Bryan Roth

    Promoter

    Syn

    Tags

    EGFP

    Available Since

    June 12, 2019

    Availability

    Academic Institutions and Nonprofits only

18. pLX313-Firefly luciferase

    Plasmid

    #118017

    Purpose

    Constitutive expression of Firefly luciferase

    Depositor

    William Hahn, David Root

    Article

    Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE

    Plasmid

    #162381

    Purpose

    AAV-mediated expression of ultrafast protein calcium sensor under the CAG promoter, Cre-dependent expression

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Has Service

    AAV Retrograde, AAV1, AAV5, and AAV9

    Insert

    jGCaMP8m
    

    Use

    AAV and Cre/Lox

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    A25G F286Y

    Promoter

    CAG

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTXB1-nbMmKappa-Tn5

    Plasmid

    #184286

    Purpose

    Anti-mouse kappa chain specific nanobody-Tn5 for NTT-seq (multiplexed CUT&Tag)

    Depositor

    New York Genome Center, Ivan Raimondi

    Article

    Stuart et al Nat Biotechnol. 2022 Dec 19. doi: 10.103

    Insert

    nbMmKappa-Tn5
    

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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