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Split-TurboID enables contact-dependent proximity labeling in cells.
Cho KF, Branon TC, Rajeev S, Svinkina T, Udeshi ND, Thoudam T, Kwak C, Rhee HW, Lee IK, Carr SA, Ting AY
Proc Natl Acad Sci U S A. 2020 May 18. pii: 1919528117. doi: 10.1073/pnas.1919528117.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
153002pLX304 CMV FKBP-V5-sTurboID (N)Expresses low-affinity split-TurboID N-terminal fragment in mammalian cells
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153003pLX304 CMV HA-HaloTag-FRB-sTurboID (C)Expresses low-affinity split-TurboID C-terminal fragment in mammalian cells
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153004pLX304 CMV FKBP-V5-sTurboID-high affinity (N)Expresses high-affinity split-TurboID N-terminal fragment in mammalian cells
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153005pLX304 CMV HA-HaloTag-FRB-sTurboID-high affinity (C)Expresses high-affinity split-TurboID C-terminal fragment in mammalian cells
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153006pLX304 CMV OMM-FKBP-V5-sTurboID (N)Expresses split-TurboID N-terminal fragment targeted to outer mitochondrial membrane
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153007pLX208 CMV sTurboID (C)-HA-FRB-ERMExpresses split-TurboID C-terminal fragment targeted to endoplasmic reticulum membrane
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153008pCAG CAG Glucagon-ICAM1-3xHA-pre-mGRASPExpresses glucagon peptide on mammalian cell surface
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153009pAAV CMV GCGR-V5-sTurboID (N)Expresses split-TuboID N-terminal fragment fused to GCGR
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153010pCDNA3 CMV sTurboID (C)-Arrestin-GFPExpresses split-TurboID C-terminal fragment fused to arrestin
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Recombinant Antibodies from Article