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pLX304 CMV HA-HaloTag-FRB-sTurboID-high affinity (C)
(Plasmid #153005)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 153005 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLX304
  • Backbone size w/o insert (bp) 7665
  • Total vector size (bp) 9657
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sTurboID-high affinity (C) (G79)
  • Insert Size (bp)
    1992
  • Promoter CMV
  • Tags / Fusion Proteins
    • HA
    • HaloTag
    • FRB

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLX304 CMV HA-HaloTag-FRB-sTurboID-high affinity (C) was a gift from Alice Ting (Addgene plasmid # 153005 ; http://n2t.net/addgene:153005 ; RRID:Addgene_153005)
  • For your References section:

    Split-TurboID enables contact-dependent proximity labeling in cells. Cho KF, Branon TC, Rajeev S, Svinkina T, Udeshi ND, Thoudam T, Kwak C, Rhee HW, Lee IK, Carr SA, Ting AY. Proc Natl Acad Sci U S A. 2020 May 18. pii: 1919528117. doi: 10.1073/pnas.1919528117. 10.1073/pnas.1919528117 PubMed 32424107