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Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effects.
Shen B, Zhang W, Zhang J, Zhou J, Wang J, Chen L, Wang L, Hodgkins A, Iyer V, Huang X, Skarnes WC
Nat Methods. 2014 Mar 2. doi: 10.1038/nmeth.2857.
PubMed Journal

Plasmids from Article

ID Plasmid  
51129 pST1374-N-NLS-flag-linker-Cas9-H840A Add to Cart
51130 ST1374-N-NLS-flag-linker-Cas9-D10A Add to Cart
51132 pUC57-sgRNA expression vector Add to Cart
51133 pGL3-U6-sgRNA-PGK-puromycin Add to Cart