PurposeWe designed a flexible linker to connect two types of anti GFP nanobodies and used this fusion nanobody to purify GFP tagged protein.
Sequences (2) — Accept Affinity Reagent Sequence Policy
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||140442||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberLow Copy
- Promoter T7 promotor
/ Fusion Protein
- 8 histidine tag (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (unknown if destroyed)
- 3′ cloning site BamHΙ (unknown if destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:N8his-GFPenhancer-GGGGS4-LaG16 was a gift from Motoyuki Hattori (Addgene plasmid # 140442 ; http://n2t.net/addgene:140442 ; RRID:Addgene_140442)
For your References section:Structure-based engineering of anti-GFP nanobody tandems as ultra-high-affinity reagents for purification. Zhang Z, Wang Y, Ding Y, Hattori M. Sci Rep. 2020 Apr 10;10(1):6239. doi: 10.1038/s41598-020-62606-7. 10.1038/s41598-020-62606-7 PubMed 32277083