PurposeExpresses GST-tagged anti-GFP Nanobody in E. coli cells.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||61838||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerGE healthcare
- Backbone size w/o insert (bp) 5000
- Total vector size (bp) 5320
Vector typeBacterial Expression
Growth in Bacteria
Insert Size (bp)355
/ Fusion Protein
- GST (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer CGTATTGAAGCTATCCCAC
- 3′ sequencing primer CGGCATCCGCTTACAGAC (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGEX6P1-GFP-Nanobody was a gift from Kazuhisa Nakayama (Addgene plasmid # 61838 ; http://n2t.net/addgene:61838 ; RRID:Addgene_61838)
For your References section:Multisubunit complex architectures revealed by visible immunoprecipitation (VIP) assay using fluorescent fusion proteins. Katoh Y, Nozaki S, Hartanto D, Miyano R, Nakayama K. J Cell Sci. 2015 May 11. pii: jcs.168740. 10.1242/jcs.168740 PubMed 25964651