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pGEX6P1-mCherry-Nanobody
(Plasmid #70696)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 70696 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGEX6P1
  • Backbone manufacturer
    GE healthcare
  • Backbone size w/o insert (bp) 5000
  • Total vector size (bp) 5371
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mCherry-Nanobody
  • Alt name
    LaM-4
  • Species
    Synthetic
  • Insert Size (bp)
    390
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CGTATTGAAGCTATCCCAC
  • 3′ sequencing primer CGGCATCCGCTTACAGAC
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    mCherry-nanobody (LaM-4) was originally developed by Dr. Rout et al. (see citation in the comments section)
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

Additional citation: Fridy PC, Li Y, Keegan S, Thompson MK, Nudelman I, Scheid JF, Oeffinger M, Nussenzweig MC, Fenyö D, Chait BT & Rout MP (2014) A robust pipeline for rapid production of versatile nanobody repertoires. Nat Meth 11: 1253–1260

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX6P1-mCherry-Nanobody was a gift from Kazuhisa Nakayama (Addgene plasmid # 70696 ; http://n2t.net/addgene:70696 ; RRID:Addgene_70696)
  • For your References section:

    Overall architecture of the intraflagellar transport (IFT)-B complex containing Cluap1/IFT38 as an essential component of the IFT-B peripheral subcomplex. Katoh Y, Terada M, Nishijima Y, Takei R, Nozaki S, Hamada H, Nakayama K. J Biol Chem. 2016 Mar 15. pii: jbc.M116.713883. 10.1074/jbc.M116.713883 PubMed 26980730