pGEX6P1-mCherry-Nanobody
(Plasmid #70696)

• Purpose: Expresses GST-tagged anti-mCherry Nanobody in E. coli cells.
• Depositing Lab: Kazuhisa Nakayama
• Publication: Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.713883.

Backbone

• Vector backbone: pGEX6P1
• Backbone manufacturer: GE healthcare
• Backbone size w/o insert (bp): 5000
• Total vector size (bp): 5371
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: mCherry-Nanobody
• Alt name: LaM-4
• Species: Synthetic
• Insert Size (bp): 390
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CGTATTGAAGCTATCCCAC
• 3′ sequencing primer: CGGCATCCGCTTACAGAC

Resource Information

• A portion of this plasmid was derived from a plasmid made by: mCherry-nanobody (LaM-4) was originally developed by Dr. Rout et al. (see citation in the comments section)
• Articles Citing this Plasmid:
  • 9 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Additional citation: Fridy PC, Li Y, Keegan S, Thompson MK, Nudelman I, Scheid JF, Oeffinger M, Nussenzweig MC, Fenyö D, Chait BT & Rout MP (2014) A robust pipeline for rapid production of versatile nanobody repertoires. Nat Meth 11: 1253–1260