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Anti-GST [N100/13R ]
(Antibody #192032)

Ordering

Item Catalog # Description Quantity Price (USD)
Recombinant Antibody 192032-rAb Limited Stock Available, 3 units left
100 µg of purified recombinant antibody
$250 *
Recombinant Antibody trial size 192032-rAb.T Limited Stock Available, 3 units left
20 µg of purified recombinant antibody
$85 *

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Features

Production & Usage

  • Production Plasmid
    Anti-GST [N100/13R ]
  • Antigen Plasmid
    pHN922
  • Purification
    Purified from cell culture supernatant using Protein A or Protein G affinity columns followed by a buffer exchange with centrifugal columns.
  • Storage Buffer
    PBS + 1 mM sodium azide

Delivery

  • Concentration
    0.9–1.1 mg/mL
  • Storage
    Upon receipt, prepare single use aliquots and store at -20 °C until use. Avoid multiple freeze thaw cycles.
  • Shipment
    Blue ice or room temp

Resource Information

  • Persistent Resource Identifier
    RRID:AB_2916235

Terms and Licenses

Target Antigen

Protein Glutathione S-transferase
Antigen Description Identified as off-target mAb in screen for anti-Stargazin Neuromabs (immunogen was GST fusion protein amino acids 204–323, cytoplasmic C-terminus, of mouse TARPGamma2). Target identified as GST by ELISA and immunoblot against other GST fusion proteins.
Species Synthetic
External References

Applications (1)

Western Blot

1 image
Western Blot image by Addgene
Submitted By: Addgene
Antibody Type
Recombinant
Sample Species
Other
Result
Pass
How to cite this antibody ( Back to top)

These antibodies were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the antibodies were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Anti-GST [N100/13R ] - from James Trimmer (Addgene antibody # 192032 ; http://n2t.net/addgene:192032 ; RRID:AB_2916235)
  • For your References section:

    High-volume hybridoma sequencing on the NeuroMabSeq platform enables efficient generation of recombinant monoclonal antibodies and scFvs for neuroscience research. Mitchell KG, Gong B, Hunter SS, Burkart-Waco D, Gavira-O'Neill CE, Templeton KM, Goethel ME, Bzymek M, MacNiven LM, Murray KD, Settles ML, Froenicke L, Trimmer JS. Sci Rep. 2023 Sep 27;13(1):16200. doi: 10.1038/s41598-023-43233-4. 10.1038/s41598-023-43233-4 PubMed 37758930