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pOPINE GFP nanobody
(Plasmid #49172)


Item Catalog # Description Quantity Price (USD)
Plasmid 49172 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    GFP nanobody
  • Alt name
    abGFP4 PMID 16095608
  • Promoter T7
  • Tag / Fusion Protein
    • HHHHHH (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (unknown if destroyed)
  • 3′ cloning site PmeI (unknown if destroyed)
  • 5′ sequencing primer T7 forward
  • 3′ sequencing primer T7 reverse
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOPINE GFP nanobody was a gift from Brett Collins (Addgene plasmid # 49172 ; ; RRID:Addgene_49172)
  • For your References section:

    Structural and thermodynamic analysis of the GFP:GFP-nanobody complex. Kubala MH, Kovtun O, Alexandrov K, Collins BM. Protein Sci. 2010 Dec;19(12):2389-401. doi: 10.1002/pro.519. 10.1002/pro.519 PubMed 20945358