Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pOPINE GFP nanobody:Halo:His6
(Plasmid #111090)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 111090 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pOPINE
  • Backbone manufacturer
    Berrow et al Nucleic Acids Res. 2007 . 35(6):e45.
  • Backbone size w/o insert (bp) 5163
  • Total vector size (bp) 6417
  • Modifications to backbone
    none
  • Vector type
    Mammalian Expression, Bacterial Expression, Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFP Nanobody
  • Alt name
    abGFP4 PMID 16095608
  • Alt name
    Kubala et al Protein Sci. 2010 Dec;19(12):2389-401. doi: 10.1002/pro.519.
  • Species
    Synthetic
  • Insert Size (bp)
    1254
  • Promoter T7 promoter
  • Tags / Fusion Proteins
    • Halo (C terminal on insert)
    • His6 (C terminal on backbone)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    GFP Nanobody; Addgene plasmid #49172 (Dr. Brett Collins) Halo tag; Promega

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Dr. Brett Collins has given his consent to making the modified GFP nanobody plasmid available at Addgene. The Halo tag can be distributed under the HaloTag Vector Limited Use Label License (Promega).

Please visit https://figshare.com/articles/Production_of_magnetic_GFP_affinity_beads/6894248 a protocol describing the production of GFP affinity beads using this plasmid.

Please visit https://www.biorxiv.org/content/early/2018/04/20/304527 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOPINE GFP nanobody:Halo:His6 was a gift from Lennart Wirthmueller (Addgene plasmid # 111090 ; http://n2t.net/addgene:111090 ; RRID:Addgene_111090)
  • For your References section:

    Nuclear Import of Arabidopsis Poly(ADP-Ribose) Polymerase 2 Is Mediated by Importin-alpha and a Nuclear Localization Sequence Located Between the Predicted SAP Domains. Chen C, Masi R, Lintermann R, Wirthmueller L. Front Plant Sci. 2018 Nov 1;9:1581. doi: 10.3389/fpls.2018.01581. eCollection 2018. 10.3389/fpls.2018.01581 PubMed 30455710