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pOPINE GFP nanobody:Halo:His6
(Plasmid #111090)


Item Catalog # Description Quantity Price (USD)
Plasmid 111090 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Berrow et al Nucleic Acids Res. 2007 . 35(6):e45.
  • Backbone size w/o insert (bp) 5163
  • Total vector size (bp) 6417
  • Modifications to backbone
  • Vector type
    Mammalian Expression, Bacterial Expression, Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    GFP Nanobody
  • Alt name
    abGFP4 PMID 16095608
  • Alt name
    Kubala et al Protein Sci. 2010 Dec;19(12):2389-401. doi: 10.1002/pro.519.
  • Species
  • Insert Size (bp)
  • Promoter T7 promoter
  • Tags / Fusion Proteins
    • Halo (C terminal on insert)
    • His6 (C terminal on backbone)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    GFP Nanobody; Addgene plasmid #49172 (Dr. Brett Collins) Halo tag; Promega
  • Article Citing this Plasmid

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Dr. Brett Collins has given his consent to making the modified GFP nanobody plasmid available at Addgene. The Halo tag can be distributed under the HaloTag Vector Limited Use Label License (Promega).

Please visit a protocol describing the production of GFP affinity beads using this plasmid.

Please visit for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOPINE GFP nanobody:Halo:His6 was a gift from Lennart Wirthmueller (Addgene plasmid # 111090 ; ; RRID:Addgene_111090)
  • For your References section:

    Nuclear Import of Arabidopsis Poly(ADP-Ribose) Polymerase 2 Is Mediated by Importin-alpha and a Nuclear Localization Sequence Located Between the Predicted SAP Domains. Chen C, Masi R, Lintermann R, Wirthmueller L. Front Plant Sci. 2018 Nov 1;9:1581. doi: 10.3389/fpls.2018.01581. eCollection 2018. 10.3389/fpls.2018.01581 PubMed 30455710